Team:Paris Liliane Bettencourt/Notebook/2011/09/07/

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Team IGEM Paris 2011

Contents

Cyrille

Miniprep and sequencing

Miniprep of:

  • pVeg-YFP TetR in pHM3 -> Sequence ok
  • TetO pVeg-YFP TetR in pHM3 -> Sequence shows that only TetO was there
  • YFP TetR BB -> SpeI site are not there in the 2 clones sequenced
  • ComS -> Ok on the two clones sequenced

Hovannes-Baptiste

Preparation of slides

Dilution of overnight cultures : PY79 (gfp-) and PY79 (gfp+) .


We waited to an OD of 0.4 (600 nm).

Two well slides : 1-control (PY79 only) 2-Mix (both strains)

Observation

-37°C Microscopy-

We observed the plate with TRANS and YFP-filter settings on the Nikon microscope. We quickly saw that our PY79 + S12 gene was not correct since we found only one cell producing GFP. We will retry the experiment tomorrow with the other -20°C glycerol for this strain, hoping it is not contaminated.

TRANS at t=0min
GFP at t=0min


We followed the plate a few hours nonetheless but no result was visible (the fluorescent cell divided only 2 times).


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