• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

Todo

Microfluidic Chips

• Continue alignment training

Preparing the parts

• Investigate failure of lysis device sequencing.

Next steps:

• Make competent cells
• Test competent cells ~10^5CFU/µg not very good
• Transform in E. Coli.
• Glycerol Stocks
• Plasmid preps
• Sequence the lysis cassette

Assembly

• Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP?
• Design Gibson primers to assemble the three different plasmids.
• Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".

Wiki

Protocols

For somebody who's made some cell cultures, please provide a sentence or two on how they're done, in the Miniprep protocol:

• Describe cell cultures in miniprep protocol

= General

• Write a new protocol!
• Write-up team presentation

Clean rooms

• Order lab notebook
• Order storage box