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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
Data
Contents |
TetR Mutants
- V36F
- P39K
- Y42F
- P39Q-Y42M
T7 Promoter Plasmids
- pSB3K1-T7-const-RFP
- pSB3K1-T7-lac-RFP
- pSB3K1-T7-lac2-RFP
- pSB3K1-T7-const-Lysis
- pSB3K1-T7-lac-Lysis
- pSB3C5-T7-const-RFP
- pSB3C5-T7-lac-RFP
- pSB3C5-T7-lac2-RFP
- pSB3C5-T7-lac-Lysis
- pSB3C5-T7-lac2-Lysis
T7 Promoter Variants
Reporter Plasmids
J61002 Ptet-RFP
TetR plasmids
pSB3K1 Pconst-TetR
Parts assembled:
- Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1 "pSB3K1"] (taken from the delivery plate)
- Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116 "j23116"] (sequence copied into our primers)
- RBS and spacer: (sequence copied into our primers)
- TetR: "TetR sequence" (623 bp) The sequence lacks a stop codon, we added TAA with our primers
Sequencing results:
Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene:"pSb3K1_TetR_seq" All these parts are correct.
pSB3K1 Pconst-TetR Ptet-LacI
Parts assembled
- Plasmid backbone containing Pconst and TetR: see precedent section
- Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014] (sequence copied into our primers)
- Ptet: R004 from Registry [http://partsregistry.org/Part:BBa_R0040] (sequence copied into our primers)
- LacI: amplified from Repressilator plasmid
Sequencing results:
