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05/19/11 Agenda:Transformation Test Run
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(Created page with "===''' 05/19/11 Agenda:Transformation Test Run'''=== (1) Obtained a vial of 2mL of TOP-10 electro competent E. coli cells 3-16-11 (44-0003/793762) (2) Added 50 microliters ...")
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(Created page with "===''' 05/19/11 Agenda:Transformation Test Run'''=== (1) Obtained a vial of 2mL of TOP-10 electro competent E. coli cells 3-16-11 (44-0003/793762) (2) Added 50 microliters ...")
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Revision as of 19:49, 2 June 2011
05/19/11 Agenda:Transformation Test Run
(1) Obtained a vial of 2mL of TOP-10 electro competent E. coli cells 3-16-11 (44-0003/793762)
(2) Added 50 microliters of BIO-RAD transformation solution (Control number 31000008916 recd 1-17-11 GT)
(3) Added 2 microliters of DNA from Registry plate 2 well 5b
(4) Incubate on ice 5 minutes.
(5) Heat shocked 40 degrees C for 50 seconds
(6) Incubated 4 degrees C for 50 seconds
(7) Added 1mL LB 5-17-11 D.G.
(8) Plate 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "who")
