Team:UNICAMP-EMSE Brazil/Notebook/31 August 2011

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Template:UNICAMP-EMSE Brazil_Notebook}} <div style="text-align: left;"> [[Image:UNICAMP-EMSE Brazil Gel1 31 08 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel...")
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*'''<font size=+5 color=teal>Results:'''</font>
*'''<font size=+5 color=teal>Results:'''</font>
<font align=center>
<font align=center>
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[[Image:UNICAMP-EMSE_Brazil_Table_temp.jpg|700px]]
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[[Image:UNICAMP-EMSE_Brazil_Table_temp.jpg|650px|center]]
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<br>
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::::<font size=+4 color=Purple align=center>'''OBS:'''</font><font size=+5 color=red> flHDC+RBS+IL 12 and  flHDC+RBS+GFP did not work! Inoculate again from a permanent culture.!</font>
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<font size=+5 color=red>flHDC+RBS+IL 12 and  flHDC+RBS+GFP did not work! Inoculate again from a permanent culture.!</font>
 
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<br>
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<br>
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<br>
[[Image:UNICAMP-EMSE Brazil Gel2 31 08 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 31/08/2011.'''</font> <br>Gel 2: HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/  SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/]]
[[Image:UNICAMP-EMSE Brazil Gel2 31 08 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 31/08/2011.'''</font> <br>Gel 2: HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/  SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/]]
===Same electrophoresys but after a longer period run:===
===Same electrophoresys but after a longer period run:===
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<font size=+4 color=teal>'''Results:'''</font>
<font size=+4 color=teal>'''Results:'''</font>
:* RBS+HlyD+TolC+Terminator has two bands, but the smaller one can be the closed vector while the larger ones can be the linearized vector. We cut the larger ones.
:* RBS+HlyD+TolC+Terminator has two bands, but the smaller one can be the closed vector while the larger ones can be the linearized vector. We cut the larger ones.
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==='''Quantification of digestion:'''===
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'''Objective:''' 
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[[Image:UNICAMP-EMSE Brazil Gel3 31 08 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 3, 31/08/2011.'''</font> <br>Gel 3: HlyA+T (E-X) 1/ HlyA+T (E-X) 2/ HlyA+T (E-X) 3/ HlyA+T (E-X) 4/ SoxS+RBS+IL10 (E-S) 1/ SoxS+RBS+IL10 (E-S) 2/ RBS+HlyD+TolC+T (E-X) 1/ RBS+HlyD+TolC+T (E-X) 2/ RBS+HlyD+TolC+T (E-X) 3/ RBS+HlyD+TolC+T (E-X) 4/ / flHDC+RBS+HlyB (E-S) 1/ flHDC+RBS+HlyB (E-S) 2/  SoxS+RBS+GFP (E-S) 1/ SoxS+RBS+GFP (E-S) 1]]
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:*Quantification of samples purified from the gel
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*'''Ladder:'''
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:*100-10.000 bp (Fermentas)
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*'''Gel Agarose concentration:'''
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:*1,0%
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:*'''Gel 3:'''
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::*HlyA+T (E-X) 1/ HlyA+T (E-X) 2/ HlyA+T (E-X) 3/ HlyA+T (E-X) 4/ SoxS+RBS+IL10 (E-S) 1/ SoxS+RBS+IL10 (E-S) 2/ RBS+HlyD+TolC+T (E-X) 1/ RBS+HlyD+TolC+T (E-X) 2/ RBS+HlyD+TolC+T (E-X) 3/ RBS+HlyD+TolC+T (E-X) 4/ / flHDC+RBS+HlyB (E-S) 1/ flHDC+RBS+HlyB (E-S) 2/  SoxS+RBS+GFP (E-S) 1/ SoxS+RBS+GFP (E-S) 1
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<br clear="all" />
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*'''<font size=+5 color=teal>Other tasks made today:'''</font>
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#Ligation of samples purified from the gel
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#Transformation and platting
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#Inoculation of fLHDC+RBS+GFP C6 from permanent culture and new colonies of fLHDC+RBS+ IL12 from the plate (C8, C9,C10,C11).
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<html>
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<img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img>
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</html>
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<html>
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<head>
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<script language='JavaScript' type='text/JavaScript'>
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function makeQuote() {
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Q = new Array();
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Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)"
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Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)"
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Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)"
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Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)"
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Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)"
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Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga"
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Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor"
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Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student"
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Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair"
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Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil"
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Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress"
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Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)"
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Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
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Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
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 +
 +
 +
 +
 +
 +
 +
 +
 +
index = Math.floor(Math.random() * Q.length);
 +
 +
document.writeln(Q[index]);
 +
 +
}
 +
 +
</script>
 +
 +
</head>
 +
 +
<style>
 +
#newquote {color:gray; font-family:verdana; font-size:11px; text-align:right; padding:5px;}
 +
 +
#newquote i {display:block; color:black; font-family:georgia, trebuchet ms; font-size:13px;}
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#newquote small {display:block; text-align:right; margin-top:10px;}
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 +
]]></b:skin>
 +
</style>
 +
</html>
 +
 +
 +
<html>
 +
<head>
 +
<div id='newquote'>
 +
 +
<script language="javascript">
 +
 +
makeQuote();
 +
 +
</script>
 +
</div>
 +
</head>
 +
</html>

Revision as of 01:27, 1 September 2011

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Contents

Notebook

Click on a date to see what we have done!

May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_May_2011&action=edit 1]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_May_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_May_2011&action=edit 8]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_May_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_May_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_May_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_May_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_May_2011&action=edit 15]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_May_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_May_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_May_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_May_2011&action=edit 22]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_May_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_May_2011&action=edit 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_May_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_May_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_May_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_May_2011 31]
June
MTWTFSS
    [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_June_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_June_2011 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_June_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_June_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_June_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_June_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_June_2011&action=edit 12]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_June_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_June_2011&action=edit 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_June_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_June_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_June_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_June_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_June_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_June_2011&action=edit 26]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_June_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_June_2011&action=edit 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_June_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_June_2011&action=edit 30]
July
MTWTFSS
        [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_July_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_July_2011&action=edit 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_July_2011 3]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_July_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_July_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_July_2011&action=edit 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_July_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_July_2011 11] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_July_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_July_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_July_2011 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_July_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_July_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/18_July_2011 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_July_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_July_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_July_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_July_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_July_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_July_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_July_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_August_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_August_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_August_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_August_2011 19] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_August_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_August_2011 21]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_August_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_August_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_August_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_August_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_August_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_August_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_August_2011&action=edit 28]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_August_2011 29] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_August_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_September_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_September_2011&action=edit 4]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/5_September_2011 5] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_September_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_September_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_September_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_September_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_September_2011 10] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_September_2011 11]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_September_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_September_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_September_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_September_2011&action=edit 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_September_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_September_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_September_2011&action=edit 18]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_September_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_September_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_September_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_September_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_September_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_September_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_September_2011 25]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_September_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_September_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_September_2011 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_September_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_October_2011&action=edit 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_October_2011 2]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_October_2011&action=edit 31]


Electrophoresis Gel 1, 31/08/2011.
Gel 1: HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/

31 August 2011

Digestion, purification and ligation!!!

Objective:

  • Digestion of constructs to purify and ligate them.

Samples:

  • Gel 1:
  • HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%


  • Results:

UNICAMP-EMSE Brazil Table temp.jpg


OBS: flHDC+RBS+IL 12 and flHDC+RBS+GFP did not work! Inoculate again from a permanent culture.!





Electrophoresis Gel 2, 31/08/2011.
Gel 2: HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/

Same electrophoresys but after a longer period run:

  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%

Samples:

  • Gel 2: Same of Gel1!
  • HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ HlyA+Terminator (E-X)/ SoxS+RBS+GFP (E-S)/ SoxS+RBS+GFP (E-S)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ RBS+HlyD+TolC+Terminator (E-X)/ SoxS+RBS+IL 10 (E-S)/ SoxS+RBS+IL 10 (E-S)/ flHDC+RBS+HlyB (E-S)/ flHDC+RBS+HlyB (E-S)/flHDC+RBS+IL 12 (E-S)/ flHDC+RBS+GFP (E-S)/

Results:

  • RBS+HlyD+TolC+Terminator has two bands, but the smaller one can be the closed vector while the larger ones can be the linearized vector. We cut the larger ones.


Quantification of digestion:

Objective:

Electrophoresis Gel 3, 31/08/2011.
Gel 3: HlyA+T (E-X) 1/ HlyA+T (E-X) 2/ HlyA+T (E-X) 3/ HlyA+T (E-X) 4/ SoxS+RBS+IL10 (E-S) 1/ SoxS+RBS+IL10 (E-S) 2/ RBS+HlyD+TolC+T (E-X) 1/ RBS+HlyD+TolC+T (E-X) 2/ RBS+HlyD+TolC+T (E-X) 3/ RBS+HlyD+TolC+T (E-X) 4/ / flHDC+RBS+HlyB (E-S) 1/ flHDC+RBS+HlyB (E-S) 2/ SoxS+RBS+GFP (E-S) 1/ SoxS+RBS+GFP (E-S) 1
  • Quantification of samples purified from the gel
  • Ladder:
  • 100-10.000 bp (Fermentas)
  • Gel Agarose concentration:
  • 1,0%
  • Gel 3:
  • HlyA+T (E-X) 1/ HlyA+T (E-X) 2/ HlyA+T (E-X) 3/ HlyA+T (E-X) 4/ SoxS+RBS+IL10 (E-S) 1/ SoxS+RBS+IL10 (E-S) 2/ RBS+HlyD+TolC+T (E-X) 1/ RBS+HlyD+TolC+T (E-X) 2/ RBS+HlyD+TolC+T (E-X) 3/ RBS+HlyD+TolC+T (E-X) 4/ / flHDC+RBS+HlyB (E-S) 1/ flHDC+RBS+HlyB (E-S) 2/ SoxS+RBS+GFP (E-S) 1/ SoxS+RBS+GFP (E-S) 1



  • Other tasks made today:
  1. Ligation of samples purified from the gel
  2. Transformation and platting
  3. Inoculation of fLHDC+RBS+GFP C6 from permanent culture and new colonies of fLHDC+RBS+ IL12 from the plate (C8, C9,C10,C11).





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