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Team:Freiburg/Notebook/30 August
From 2011.igem.org
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SophieCramer (Talk | contribs) (→Cloning) |
SophieCramer (Talk | contribs) (→Precipitator) |
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==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
| - | '''Digestion''' | + | '''Digestion''' this time the vector is dephosphorilated by antarctic phosphatase to avoid religation. |
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# 1 μl restriction enzymes (stored at iGEM’s, -20°C) | # 1 μl restriction enzymes (stored at iGEM’s, -20°C) | ||
# heat for 1-2 hours 37°C (6 hours if time) | # heat for 1-2 hours 37°C (6 hours if time) | ||
| + | '''# add 5,6 μl antarctic phosphatase buffer and 1 μl antarctic phosphatase and incubate another hour''' | ||
# heat for 20 minutes 80°C (inactivation of enzymes) | # heat for 20 minutes 80°C (inactivation of enzymes) | ||
# keep at 4°C if you cannot continue | # keep at 4°C if you cannot continue | ||
Revision as of 15:28, 31 August 2011
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