Team:Paris Bettencourt/Modeling/Parameters

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Revision as of 11:57, 30 August 2011

Team IGEM Paris 2011

Parameters

Parameter Description Value Unit Reference
Concentration of X NA molecules
per cell		 Notation convention
Delay due to protein X production and maturation NA s Notation convention
Maximal production rate of pVeg promoter (constitutive) ??? molecules.s-1
or pops		 Estimated
Maximal production rate of pLac promoter 0.02 molecules.s-1
or pops		 Estimated
Maximal production rate of pT7 promoter 0.02 molecules.s-1
or pops		 Estimated
Dissociation constant for IPTG to LacI 1200 molecules
per cell		 Aberdeen 2009 wiki
Dissociation constant for LacI to LacO (pLac) 700 molecules
per cell		 Aberdeen 2009 wiki
Dissociation constant for T7 RNA polymerase to pT7 3 molecules
per cell		 Estimated ADD EXPLANATION
Translation rate of proteins 1 or 0.9? s-1 Estimated ADD EXPLANATION
Dilution rate in exponential phase 2.88x10-4 s-1 Calculated with a 40 min generation time. See explanation
Degradation rate of mRNA 2.88x10-3 s-1 Uri Alon (To Be Confirmed)
Delay due tT7 RNA polymerase production and maturation 300 s http://mol-biol4masters.masters.grkraj.org/html/Prokaryotic_DNA_Replication13-T7_Phage_DNA_Replication.htm
Delay due to mRNA production 30 s http://bionumbers.hms.harvard.edu/bionumber.aspx?s=y&id=104902&ver=5&hlid=58815 2kb/(50b/s) --> approximation: all our contructs are around 2kb

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