Revision as of 15:50, 25 August 2011

PROJECT

RESULTS

CONSIDERATIONS

ABOUT US

NOTEBOOK

ATTRIBUTIONS

File:Protocols img1.gif

Cloning

|| Transformation || Overnight || Miniprep || 3A Assembly ||
|| PCR Amplification || PCR Purification ||

File:Protocols img22.jpg

Primers and Design

|| Primer Design || Oligo Annealing || DNA Sequencing ||
|| Linearized Plasmid Backbones ||

Cells and Solutions

|| Top10 Chemically Competent Cells || CCMB80 Buffer ||
|| Media || Glycerol Stocks || Agarose Gels || Plates ||
|| Antibiotic Stocks ||

@@ Line 13: / Line 13: @@
 [[Team:Northwestern/Notebook/Protocols/Miniprep| Miniprep]] ||
 [[Team:Northwestern/Notebook/Protocols/3A_Assembly| 3A Assembly]] ||<br />
-[[Team:Northwestern/Notebook/Protocols/PCR_Amplification| PCR Amplification]] ||
+|| [[Team:Northwestern/Notebook/Protocols/PCR_Amplification| PCR Amplification]] ||
-[[Team:Northwestern/Notebook/Protocols/PCR_Purification| PCR Purification]]
+[[Team:Northwestern/Notebook/Protocols/PCR_Purification| PCR Purification]] ||
 </div>
 </div>
@@ Line 27: / Line 27: @@
 <div style="margin:10px 0px 0px 150px;color:#ffffff;font:28px helvetica;font-weight:25;">
 &nbsp;'''Primers and Design''' <br /></div><div style="margin:5px 0px 0px 240px;color:#ffffff">
-[[Team:Northwestern/Notebook/Protocols/Primer_Design| Primer Design]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Primer_Design| Primer Design]] ||
 [[Team:Northwestern/Notebook/Protocols/Oligo_Annealing| Oligo Annealing]] ||
-[[Team:Northwestern/Notebook/Protocols/Sequencing| DNA Sequencing]] ||
+[[Team:Northwestern/Notebook/Protocols/Sequencing| DNA Sequencing]] ||<br />
-[[Team:Northwestern/Notebook/Protocols/Linearized_Plasmid_Backbones| Linearized Plasmid Backbones]]
+|| [[Team:Northwestern/Notebook/Protocols/Linearized_Plasmid_Backbones| Linearized Plasmid Backbones]] ||
 </div>
 </div>
@@ Line 44: / Line 44: @@
 <div style="margin:10px 0px 0px 150px;color:#ffffff;font:28px helvetica;font-weight:25;">
 &nbsp;'''Cells and Solutions''' <br /></div><div style="margin:5px 0px 0px 240px;color:#ffffff">
-[[Team:Northwestern/Notebook/Protocols/Top10_Competent| Top10 Chemically Competent Cells]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Top10_Competent| Top10 Chemically Competent Cells]] ||
-[[Team:Northwestern/Notebook/Protocols/CCMB80| CCMB80 Buffer]] ||
+[[Team:Northwestern/Notebook/Protocols/CCMB80| CCMB80 Buffer]] ||<br />
-[[Team:Northwestern/Notebook/Protocols/Media| Media]] ||
+|| [[Team:Northwestern/Notebook/Protocols/Media| Media]] ||
 [[Team:Northwestern/Notebook/Protocols/Glycerol| Glycerol Stocks]] ||
 [[Team:Northwestern/Notebook/Protocols/Agarose_Gels| Agarose Gels]] ||
-[[Team:Northwestern/Notebook/Protocols/Antibiotic_Stocks| Antibiotic Stocks]] ||
+[[Team:Northwestern/Notebook/Protocols/Plates| Plates]] ||<br />
-[[Team:Northwestern/Notebook/Protocols/Plates| Plates]]
+||[[Team:Northwestern/Notebook/Protocols/Antibiotic_Stocks| Antibiotic Stocks]] ||
 </div>
 </div>
 <!-- ----------------------------------------------------------- -->