Team:EPF-Lausanne/Notebook/August2011

From 2011.igem.org

(Difference between revisions)
(Saturday, 20 August)
(Saturday, 20 August)
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Since the previous day's Colony PCR had partly failed due to an extension time for lysis that was too short (1 minute rather than 2 minutes), Vincent made a Colony PCR for K1-T7-Lysis and a Colony PCR for C5-T7-RFP and C5-T7-Lysis. Perhaps because it was almost 9 in the evening, Vincent did not realize that he had successfully amplified both the K1 and the C5 T7 lysis, showing that the Gibson Assembly had in fact worked as expected.  
Since the previous day's Colony PCR had partly failed due to an extension time for lysis that was too short (1 minute rather than 2 minutes), Vincent made a Colony PCR for K1-T7-Lysis and a Colony PCR for C5-T7-RFP and C5-T7-Lysis. Perhaps because it was almost 9 in the evening, Vincent did not realize that he had successfully amplified both the K1 and the C5 T7 lysis, showing that the Gibson Assembly had in fact worked as expected.  
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[[File:Vince_gel_C5rfp.jpg|600px]]
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[[File:vince_gel_C5rfp.jpg|600px]]
== Monday, 22 August 2011 ==
== Monday, 22 August 2011 ==

Revision as of 07:40, 25 August 2011