"
Copenhagen/23 August 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "==Tuesday== The expression culture grows fine. ===Lab Work=== * Samples of the expression culture for A1 and the control with the original A1 have been taken out twice today...") |
(→Other Work) |
||
| Line 19: | Line 19: | ||
* We have worked on the project description. | * We have worked on the project description. | ||
| - | * Ordered primers for mutation of CYP2C9 - the | + | * Ordered primers for mutation of CYP2C9 - the ordinary way and the user way. |
* Ordered primers for assambly of our BioBrick in an expression vector and in a shipping plasmid. | * Ordered primers for assambly of our BioBrick in an expression vector and in a shipping plasmid. | ||
Latest revision as of 07:28, 24 August 2011
Tuesday
The expression culture grows fine.
Lab Work
- Samples of the expression culture for A1 and the control with the original A1 have been taken out twice today.
- Mini prep of R0010 promoter and our BioBricks 2*A1 and 2*a2 and RBS B0030 and BB0034.
- Double digestion of the promoter with PstI and SpeI and the RBS with PstI and Xba in 37 degrees overnight
- Sent our BioBricks to be sequenced.
Other Work
- We have worked on the project description.
- Ordered primers for mutation of CYP2C9 - the ordinary way and the user way.
- Ordered primers for assambly of our BioBrick in an expression vector and in a shipping plasmid.
Back to notebook
