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Team:UNICAMP-EMSE Brazil/protocols/Digestion
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Revision as of 20:46, 22 August 2011
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Digestion
(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction)
Use 3000 ng BioBrick vector 6 µL 10x buffer 0.5 µL enzyme 1 (20 units/µL) 0.5 µL enzyme 2 (20 units/µL) distilled water to 60 µL total volume Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.
Make an electrophoresys (use the hole reaction volume) and cut the band with your gene of interest.
