Team:EPF-Lausanne/Notebook/August2011

From 2011.igem.org

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(Friday, 19 August 2011)
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# PQ39YM42 variants:  [https://static.igem.org/mediawiki/2011/6/68/PQ39YM42LP52_BP5col6.pdf "PQ39YM42LP52"]  [https://static.igem.org/mediawiki/2011/6/6c/PQ39YM42_BP5col4_insertion_deletion.pdf "PQ39YM42_ins_del"]   
# PQ39YM42 variants:  [https://static.igem.org/mediawiki/2011/6/68/PQ39YM42LP52_BP5col6.pdf "PQ39YM42LP52"]  [https://static.igem.org/mediawiki/2011/6/6c/PQ39YM42_BP5col4_insertion_deletion.pdf "PQ39YM42_ins_del"]   
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== Monday, 22 August 2011 ==
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Nadine made 2 Gibson reactions (equimolar ratios):
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* LacI and pSB3K1 Pconst-TetR, to assemble pSB3K1 Pconst-TetR Ptet-LacI plasmid
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* RFP and J61002 backbone, to assemble J61002 Plac-RFP plasmid
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She also made negative controls, where only the backbone has been mixed with water.
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Revision as of 09:40, 22 August 2011