Team:UNICAMP-EMSE Brazil/Notebook/20 August 2011
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*Ladder: 100-10.000 bp (Fermentas) | *Ladder: 100-10.000 bp (Fermentas) | ||
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- | *'''Picture: Upper part:''' | + | *'''Picture legend: Upper part:''' |
#'''Gel 1:''' flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D) | #'''Gel 1:''' flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D) | ||
#'''Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D) | #'''Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D) |
Revision as of 20:42, 21 August 2011
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Notebook
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20 August 2011
Everybody:
Objective: Check if the size of the synthetic DNA sequences that didn´t work on 19/08/2011.
- Agarose: 0,8%
- Ladder: 100-10.000 bp (Fermentas)
- Picture legend: Upper part:
- Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D)
- Gel 2: / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)
Nd: non digested
D: digested
D: digested