Team:UT Dallas/NotebookWeek2

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" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
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       <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
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       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>

Revision as of 15:10, 17 August 2011

biz solution

Week 2

July 18-

  • Colony grew, negative control didn’t
  • Transfomation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all carb)
  • Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol
  • Miniprep
  • Dissolving and diluting primers (10 pmol/µL)
  • PCR and gel electrophoresis and purification
  • Digestion for 1 hour and 30 minutes at 37 degrees Celsius
  • Gel Electrophoresis and Purification
  • Digestion and ligation
  • July 19-

  • Transformation- 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight
  • July 20-

  • Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature
  • Transformation of Photo and YFP 50 µL DH5α, 2 µL DNA at 37 degrees Celsius overnight (all chlora)
  • Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513
  • July 21-

  • Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • Test digestion for 1 hour and 20 minutes a 37 degrees Celsius
  • - Test digestion for 1 hour and 20 minutes a 37 degrees Celsius
  • July 22-

  • Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells
  • Miniprep
  • Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol
  • Dissolving and Diluting primers
  • Test digestion and gel electrophoresis
  • Image Gallery

    Notebook

    Learn more...