Team:UT Dallas/NotebookWeek1

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       <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
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       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>

Latest revision as of 15:10, 17 August 2011

biz solution

Week 1

July 11-

  • Preparing sent parts
  • July 12-

  • Preparing sent parts
  • growing LB and appropriate antibiotic overnight
  • July 13-

  • all grew, negative controls didn't
  • Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic
  • July 14-

  • all grew a blob and a few colonies along the edge
  • - Transformation at 37 degrees Celsius overnight- 50 µL DH5α, 2 µL DNA (all carb)
  • Plating sent parts
  • Take one colony from July 13 plate properly
  • July 15-

  • Transformation of 50µL of DH5α, 2 µL DNA (all carb) at 37 degrees overnight
  • Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
  • July 16-

  • All but negative control grew 12.2.13 is slightly pink probably due to the constant red
  • Glycerol stock- 350 µL of cells and 150 µL if 50% glyrcerol
  • Miniprep and transformation results- the plate had about 50 to 60 colonies
  • July 17-

  • Incubate in 3 mL LB broth overnight at 37 degrees Celsius and 2020 rpm
  • Image Gallery

    Notebook

    Learn more...