Team:Wageningen UR/Notebook/Proj2/March

From 2011.igem.org

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  <div><a name="March8">March 8</a></div>  
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<span style="color:white; text-align:justify;"> The pathways have been modelled in Tinkercell. Research has to be done to find orthologs in <span style="font-style:italic;">Aspergillus</span> and to make sure the genes we want to use are really present in <span style="font-style:italic;">Aspergillus</span>. A decision has to be made about what kind of selection markers we are going to use. We are looking for an RFP variant which can be functionally expressed in <span style="font-style:italic;">Aspergillus</span>. </span>
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<span style="color:white; text-align:justify;"> We might have a problem: the mechanism might not work, because there is small leakage from the leu3p repressing system which isn’t wanted because we have an autoinducing system. We have to contact our supervisors about it and talk about it how big the problem is. </span>
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<div><a name="March15">March 17</a></div>
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<span style="color:white; text-align:justify;"> test </span>
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  <div><a name="March22">March 22</a></div>  
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<span style="color:white; text-align:justify;"> The list of the genes is almost finished! Next step: making a BioBrick Design. </span>
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  <div><a name="March29">March 29</a></div>  
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<span style="color:white; text-align:justify;"> There was some debate about whether we should work with <span style="font-style:italic;">Aspergillus nidulans</span> or <span style="font-style:italic;">Aspergillus niger</span>. <span style="font-style:italic;">Aspergillus nidulans</span> is decided to be the best choice. </span>
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Revision as of 12:45, 23 May 2011


March - Fungal Track 'n Trace

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Mar2.png





March 8
The pathways have been modelled in Tinkercell. Research has to be done to find orthologs in Aspergillus and to make sure the genes we want to use are really present in Aspergillus. A decision has to be made about what kind of selection markers we are going to use. We are looking for an RFP variant which can be functionally expressed in Aspergillus.


March 15
We might have a problem: the mechanism might not work, because there is small leakage from the leu3p repressing system which isn’t wanted because we have an autoinducing system. We have to contact our supervisors about it and talk about it how big the problem is.


March 22
The list of the genes is almost finished! Next step: making a BioBrick Design.


March 29
There was some debate about whether we should work with Aspergillus nidulans or Aspergillus niger. Aspergillus nidulans is decided to be the best choice.