Team:EPF-Lausanne/Notebook/August2011

From 2011.igem.org

(Difference between revisions)
(Friday, 12 August 2011)
(Monday, 15 August 2011)
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== Monday, 15 August 2011 ==
== Monday, 15 August 2011 ==
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Alessandro ran the gel for the extension PCR on RFP and for gene-specific PCR on T4.
 
All the LB bottles were contaminated therefore Alessandro made new ones. By now just one bottle at a time should be used by iGEMers in order to avoid multiple contamination.
All the LB bottles were contaminated therefore Alessandro made new ones. By now just one bottle at a time should be used by iGEMers in order to avoid multiple contamination.
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Alessandro ran the gel for the extension PCR on RFP and for gene-specific PCR on T4. In the picture there's the result: we miss a signal from T7lac-RFP and we have a weak signal from T7lac2-RFP therefore these two reactions have been repeated.
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Alessandro also made PCRs to amplify pSB3K1 and pSB3C5 plasmids to use them as backbone for our device (T7-lysis/RFP).
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Revision as of 13:56, 15 August 2011