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Team:EPF-Lausanne/Protocols/T7-ext
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(Created page with "{{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=T7 extension PCR}} This protocols consists in 3 steps: * Gene-specific PCR: amplifies the gene you want to put under the cont...")
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Revision as of 12:52, 15 August 2011
T7 extension PCR
Back to protocols.This protocols consists in 3 steps:
- Gene-specific PCR: amplifies the gene you want to put under the control of the T7 promoter adding RBS upstream and overhangs for the next PCR
- Extension PCR: adds the T7 promoter (or its variants) upstream the gene and overhangs for the Gibson assembly
- Final PCR: amplifies the fragments extending the gibson overhangs
