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Team:EPF-Lausanne/Protocols/Primers preparation
From 2011.igem.org
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| - | When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to: | + | When receiving new primers, we need to add water in them on order to achieve '''50 microM''' concentration. One rapid method is to: |
* take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | * take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water | ||
* '''spin down the primers tube''' to be sure that all DNA is at the bottom | * '''spin down the primers tube''' to be sure that all DNA is at the bottom | ||
Revision as of 12:42, 15 August 2011
Initial primer dilution
Back to protocols.
When receiving new primers, we need to add water in them on order to achieve 50 microM concentration. One rapid method is to:
- take the value "nmol yield" on the synthesis report and multiply it 20x to have the needed microliters of water
- spin down the primers tube to be sure that all DNA is at the bottom
- add the calculated amount of water in each tube, then mix them
