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Team:Macquarie Australia/Notebook
From 2011.igem.org
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. | You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. | ||
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| + | -LB media Method- | ||
| + | Dissolve 10g trytone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, bring volume up to 1 litre using the MilliQ water. Autoclave 500 mL of the solution (121oC, 15 min, standard liquid cycle). | ||
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| + | -LB agar Method- | ||
| + | Add 7.5 g Bacto agar to the remaining 500 mL of LB media and autoclave [121oC, 15 min, standard liquid cycle]. Add 250 µL of Chloroamphenicol and mix well before plating out and setting agar. | ||
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Revision as of 02:34, 14 August 2011
Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
-LB media Method- Dissolve 10g trytone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, bring volume up to 1 litre using the MilliQ water. Autoclave 500 mL of the solution (121oC, 15 min, standard liquid cycle).
-LB agar Method- Add 7.5 g Bacto agar to the remaining 500 mL of LB media and autoclave [121oC, 15 min, standard liquid cycle]. Add 250 µL of Chloroamphenicol and mix well before plating out and setting agar.
