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Team:Freiburg/Notebook/11 August
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===NAME OF YOUR EXPERIMENT: Tramsformation with inducible Promoter=== | ===NAME OF YOUR EXPERIMENT: Tramsformation with inducible Promoter=== | ||
| - | '''Investigators: Sophie'' | + | '''Investigators: Sophie''' |
We didn't get any clones with both Plastic binding domain and Promoter-RBS inside, the Pbd seems to be toxic when expressed.So we want to try, if it is possible to get cells with an inducible Promoter and Pbd. In this case we could cultivate the cells and induce expression of the Pbd only shortly before lysis. As our Light-inducible Promoters are not finished we use the IPTG inducible Promoter BBa_J04500 (Plate4, 12A) | We didn't get any clones with both Plastic binding domain and Promoter-RBS inside, the Pbd seems to be toxic when expressed.So we want to try, if it is possible to get cells with an inducible Promoter and Pbd. In this case we could cultivate the cells and induce expression of the Pbd only shortly before lysis. As our Light-inducible Promoters are not finished we use the IPTG inducible Promoter BBa_J04500 (Plate4, 12A) | ||
{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Revision as of 15:59, 11 August 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
