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Team:EPF-Lausanne/Tools
From 2011.igem.org
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For the in vivo and in vitro characterization of our mutant transcription factors, we used two high-troughput microfluidic devices, a cell culture chip and respectively a fluidic platform for measuring molecular binding events, called "MITOMI". | For the in vivo and in vitro characterization of our mutant transcription factors, we used two high-troughput microfluidic devices, a cell culture chip and respectively a fluidic platform for measuring molecular binding events, called "MITOMI". | ||
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| + | The field of microfluidics has as an objective the manipulation and analysis of fluids | ||
| + | within micrometer-sized channels, which, due to the small size, must be microfabricated using | ||
| + | techniques adapted from the semiconductor and plastics industries. | ||
| + | |||
| + | The advantages of this technique include: | ||
| + | |||
| + | * low sample and reagent volumes leading to lower costs low waste levels | ||
| + | * unique physical properties of microscale fluid flow | ||
| + | * the reduction of the size of equipment | ||
| + | * fast analysis and short reaction times | ||
| + | * parallel operation for multiple analyses | ||
| + | * possibility of portable devices. | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
Latest revision as of 19:35, 7 August 2011
Microfluidics-work in progress
For the in vivo and in vitro characterization of our mutant transcription factors, we used two high-troughput microfluidic devices, a cell culture chip and respectively a fluidic platform for measuring molecular binding events, called "MITOMI".
The field of microfluidics has as an objective the manipulation and analysis of fluids within micrometer-sized channels, which, due to the small size, must be microfabricated using techniques adapted from the semiconductor and plastics industries.
The advantages of this technique include:
- low sample and reagent volumes leading to lower costs low waste levels
- unique physical properties of microscale fluid flow
- the reduction of the size of equipment
- fast analysis and short reaction times
- parallel operation for multiple analyses
- possibility of portable devices.
