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Team:Paris Liliane Bettencourt/Notebook/2011/07/19/
From 2011.igem.org
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(Created page with "<p style="display:block;"><font color="red">Edward</font> </p><p style="display:block;"><u>Minipreps</u> </p> <ul><li>on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL </li...") |
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[[File:T7 electro 19072011.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]] | [[File:T7 electro 19072011.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]] | ||
[[File:T7 electro 19072011cut.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]] | [[File:T7 electro 19072011cut.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7 ]] | ||
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| + | <u>DNA Gel extraction (T7 RNA polymerase)</u> | ||
| + | <ul><li>BBa_K145001 cut by XbaI and PstI | ||
Revision as of 14:28, 4 August 2011
Edward
Minipreps
- on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
- on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
- on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL
Restriction Digest of RBS-spoVg and T7 RNA polymerase
- BBa_K145001 cut by XbaI and PstI
- BBa_K143021 cut by SpeI and PstI
Final volume : 20 µLDNA Gel electrophoresis (T7 RNA polymerase)
- 1kb ladder
- T7 undigested plasmid
- Digested T7 (BBa_K145001 cut by XbaI and PstI)
ladder - non digested T7 plasmid - digested T7
ladder - non digested T7 plasmid - digested T7
DNA Gel extraction (T7 RNA polymerase)- BBa_K145001 cut by XbaI and PstI
