Team:Paris Liliane Bettencourt/Notebook/2011/07/19/

From 2011.igem.org

Team IGEM Paris 2011

Edward

Minipreps

  • BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
  • BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
  • BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL


    Restriction Digest of RBS-spoVg and T7 RNA polymerase

    • BBa_K145001 cut by XbaI and PstI
    • BBa_K143021 cut by SpeI and PstI


      Final volume : 20 µL

      DNA Gel electrophoresis (T7 RNA polymerase)

      • 1kb ladder
      • T7 undigested plasmid
      • Digested T7 (BBa_K145001 cut by XbaI and PstI)
      ladder - non digested T7 plasmid - digested T7
      ladder - non digested T7 plasmid - digested T7


      DNA Gel extraction (T7 RNA polymerase)

      • BBa_K145001 cut by XbaI and PstI

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