"
Copenhagen/4 August 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "==Thursday== ''Lab Work'' * Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday. * Do...") |
(→Thursday) |
||
| Line 16: | Line 16: | ||
* Studied on USER cloning and primer design. | * Studied on USER cloning and primer design. | ||
| + | |||
| + | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 13:21, 4 August 2011
Thursday
Lab Work
- Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday.
- Double digest of 2.23.3 and 2.23.4 (purple)follwed by gelelectroforesis, byt it seems like, they digest havn't worked, so this step has to be done again.
- Transformation of A1,A2/Exp. vector into BL21 cells and A2,B1/pSB1C3 into XLblue cells.
Other Work
- Studied on the requirements for the characterization of the BioBricks.
- Meeting with Michael Lyngkjær about inducible E.coli promoters.
- Studied on USER cloning and primer design.
Back to Notebook
