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Team:EPF-Lausanne/Protocols
From 2011.igem.org
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| - | PDMS 2 layer device fabrication | + | ==PDMS 2 layer device fabrication ==16.05.2011 |
| - | Materials: | + | "Materials": |
• flow and control layer molds | • flow and control layer molds | ||
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• TMCS | • TMCS | ||
| - | Method: | + | "Method": |
• Place molds into a TMCS vapor chamber | • Place molds into a TMCS vapor chamber | ||
Revision as of 09:17, 16 May 2011
==PDMS 2 layer device fabrication ==16.05.2011
"Materials":
• flow and control layer molds • Sylgard 184 silicone elastomer kit: Base part and a Curing agent • TMCS
"Method":
• Place molds into a TMCS vapor chamber • Control layer mixture: 20g Base + 4g Curing agent • Mix for 1 minute, degas for 2 minutes (standard protocol) • pour onto control layer mold and place mold in vacuum chamber • Flow layer mixture: 20g Base + 1g Curing agent • Mix for 1 minute and degas for 2 minutes (standard protocol) • Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater) • Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some ) • Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!) • Remove casts from oven, cut out control layer, punch holes, and align to flow layer • Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing) • Remove devices from oven and punch flow layer holes
