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Team:Sevilla/Week2
From 2011.igem.org
Elena moreno (Talk | contribs) |
Elena moreno (Talk | contribs) |
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| Line 150: | Line 150: | ||
<p> | <p> | ||
| - | Our inoculums look pleasantly cloudy, as expected. All except two. We freeze samples of the good ones in glycerol at - | + | Our inoculums look pleasantly cloudy, as expected. All except two. We freeze samples |
| - | </p> | + | of the good ones in glycerol at -80 ºC in a wonderful fridge that could also easily house |
| + | a corpse (who knows?).</p> | ||
<p> | <p> | ||
| - | We start studying the protocols of our | + | We start studying the protocols of our mini-prep kit to purify the BioBrick plasmids |
| + | from our transformed cultures. They don't look that complicated but we have to do | ||
| + | about 50; shoulders to the wheel! | ||
</p> | </p> | ||
<p> | <p> | ||
| - | We receive several BioBricks and strains we had ordered | + | We receive several BioBricks and the strains we had ordered: vainillin and rhlR |
| - | </p> | + | BioBricks, two strains of B.subtilis, another strain containing VanM/N genes... people |
| + | are being quite kind to us! We seed the bacteria and transform them with the BioBricks, | ||
| + | depending on the format they come in.</p> | ||
<p> | <p> | ||
| - | Our | + | Our mini-preps are ready at last after a hard day’s work, and we are able to leave some |
| + | inoculums of DH5-a to prepare competent cells tomorrow.</p> | ||
| + | <p> | ||
| + | 21.30: Lab OFF. | ||
| + | |||
</p> | </p> | ||
| Line 198: | Line 207: | ||
<p> | <p> | ||
| - | Materials and chemicals keep | + | Materials and chemicals keep appearing in big cardboard boxes full of styro-foam |
| + | pieces (we're storing it all in the hopes of filling a bath next month and having some | ||
| + | fun). | ||
| + | |||
</p> | </p> | ||
<img src="https://lh6.googleusercontent.com/-rGz7VRV5D7s/ThdO7FOGLsI/AAAAAAAAAO8/hpt5llffrx0/s288/IMG_4499.JPG" style=padding-left:200px;"height="192" width="288" /><img src="https://lh5.googleusercontent.com/-ZxtQGA3Yc7U/TiWW4CRrSoI/AAAAAAAAARA/Rv1v4GuDp5A/s288/IMG_4648.JPG" style=padding-left:20px;"height="192" width="288" /> | <img src="https://lh6.googleusercontent.com/-rGz7VRV5D7s/ThdO7FOGLsI/AAAAAAAAAO8/hpt5llffrx0/s288/IMG_4499.JPG" style=padding-left:200px;"height="192" width="288" /><img src="https://lh5.googleusercontent.com/-ZxtQGA3Yc7U/TiWW4CRrSoI/AAAAAAAAARA/Rv1v4GuDp5A/s288/IMG_4648.JPG" style=padding-left:20px;"height="192" width="288" /> | ||
<p> | <p> | ||
| - | More good news: our transformed cultures have grown as expected, as | + | More good news: our transformed cultures have grown as expected, as have the B. |
| + | subtilis strains. At the end of the day we make inoculums of them all so that they grow | ||
| + | o/n and tomorrow we can freeze samples and do yet more mini-preps (we have only just | ||
| + | started and we're already sick and tired of that kit...) | ||
| + | |||
</p> | </p> | ||
<p> | <p> | ||
| - | We need to discuss which assembling method we'll use with the BioBricks. It takes us some hours, and in the | + | We need to discuss which assembling method we'll use with the BioBricks. It takes |
| + | us some hours, and in the meantime, Andrés faces the personal challenge of making | ||
| + | competent cells from the DH5-a inoculum with the new protocol. Tomorrow we'll see | ||
| + | who has won the battle... | ||
| + | |||
</p> | </p> | ||
<p> | <p> | ||
| - | And Pedro isn't wasting time at all, he's just found out racks are stackable | + | And Pedro isn't wasting any time at all, he's just found out these racks are stackable, like |
| + | Lego. This discovery has changed his life, look how happy he is! | ||
| + | |||
</p> | </p> | ||
<img src="https://lh5.googleusercontent.com/-xkIfQD6E3Ok/TiWXB1_8s2I/AAAAAAAAASc/vEdT33by8WM/s288/IMG_4670.JPG" style=padding-left:300px;"height="192" width="288" /> | <img src="https://lh5.googleusercontent.com/-xkIfQD6E3Ok/TiWXB1_8s2I/AAAAAAAAASc/vEdT33by8WM/s288/IMG_4670.JPG" style=padding-left:300px;"height="192" width="288" /> | ||
<p> | <p> | ||
| - | After deciding how we'll build each BioBrick and counting all the ligations we need to do, we finally tackle the digestions with the different restriction enzymes. Mastermix kits. More protocols. GREAT. | + | After deciding how we'll build each BioBrick, and counting all the ligations we need to |
| + | do, we finally tackle the digestions with the different restriction enzymes. Mastermix | ||
| + | kits this time. More protocols. GREAT. | ||
| + | |||
</p> | </p> | ||
<input type="button" value="Show Tuesday protocols" id="tuesdayBP"> | <input type="button" value="Show Tuesday protocols" id="tuesdayBP"> | ||
| Line 248: | Line 273: | ||
<p> | <p> | ||
| - | AT LAST our official mascot is here! Say hello to Mr. | + | AT LAST our official mascot is here! Say hello to Mr. Gibbs! Rocío had him held |
| + | captive in her house, but to make up for that, she's brought a WONDERFUL Disney | ||
| + | princess clock to cheer our lab up! It gives the lab that homely touch. We have also | ||
| + | brought a coffee machine - we need caffeine in industrial amounts to work here for so | ||
| + | many hours a day. | ||
| + | </p> | ||
<img src="https://lh5.googleusercontent.com/-GXrWkcwPJPU/TibGABAC11I/AAAAAAAAAVs/jg8cex8tdHk/s288/IMG_4711.JPG" style=padding-left:200px;"height="288" width="192" /> <img src="https://lh5.googleusercontent.com/-KRWFbZ4F2Ms/Tif3xgtvNaI/AAAAAAAAAY8/H2PLNUHJnDY/s400/IMG_4755.JPG" height="267" width="400" /> | <img src="https://lh5.googleusercontent.com/-GXrWkcwPJPU/TibGABAC11I/AAAAAAAAAVs/jg8cex8tdHk/s288/IMG_4711.JPG" style=padding-left:200px;"height="288" width="192" /> <img src="https://lh5.googleusercontent.com/-KRWFbZ4F2Ms/Tif3xgtvNaI/AAAAAAAAAY8/H2PLNUHJnDY/s400/IMG_4755.JPG" height="267" width="400" /> | ||
<p> | <p> | ||
| - | More | + | |
| + | More mini-preps and frozen samples. But we're also trying something new! Our first | ||
| + | electrophoresis to see how the digestions and the mini-preps go. We're examining the | ||
| + | results. It's ok for the first time, but there's a long way to go yet... | ||
</p> | </p> | ||
<p> | <p> | ||
| Line 258: | Line 291: | ||
<p> | <p> | ||
...and how did the great battle end? Andrés 1 - E.coli 0. We have competent cells! | ...and how did the great battle end? Andrés 1 - E.coli 0. We have competent cells! | ||
| + | </p> | ||
| + | <p> | ||
| + | Out of the lab before 19.00. What a record! | ||
| + | |||
</p> | </p> | ||
| Line 296: | Line 333: | ||
<p> | <p> | ||
| - | We run an electrophoresis of the digestions we made yesterday, but it hasn't worked | + | We run an electrophoresis of the digestions we made yesterday, but it hasn't worked properly, it seems the process still needs some fine-tuning. |
</p> | </p> | ||
<p> | <p> | ||
| - | We spend some time preparing the rewards for those who gave us money for the project through the | + | We spend some time preparing the rewards for those who gave us money for the project |
| - | Letters, funny badges, videos... | + | through the crowd-funding platform. Letters, funny badges, videos... |
</p> | </p> | ||
<p> | <p> | ||
| Line 307: | Line 344: | ||
<img src="https://lh3.googleusercontent.com/-S90vyEr0ke8/Tif3HI93rhI/AAAAAAAAAXg/LSvK9aosDRA/s400/IMG_4732.JPG" style=padding-left:200px;" height="267" width="400" /> | <img src="https://lh3.googleusercontent.com/-S90vyEr0ke8/Tif3HI93rhI/AAAAAAAAAXg/LSvK9aosDRA/s400/IMG_4732.JPG" style=padding-left:200px;" height="267" width="400" /> | ||
<p> | <p> | ||
| - | We have a nice visit today! Lauren, from the team of | + | We have a nice visit today! Lauren, from the team of Pennsylvania, is here to tell us |
| + | about her project, and learn a bit more about ours. Carlos is having an intense discussion | ||
| + | on kinetics with her while Marta struggles to design the primers we'll need for the PCR | ||
| + | reactions next week. There's no way to get them exactly right, what a nightmare! | ||
| + | |||
</p> | </p> | ||
Revision as of 14:32, 27 July 2011
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Monday 18 July
Our inoculums look pleasantly cloudy, as expected. All except two. We freeze samples of the good ones in glycerol at -80 ºC in a wonderful fridge that could also easily house a corpse (who knows?).
We start studying the protocols of our mini-prep kit to purify the BioBrick plasmids from our transformed cultures. They don't look that complicated but we have to do about 50; shoulders to the wheel!
We receive several BioBricks and the strains we had ordered: vainillin and rhlR BioBricks, two strains of B.subtilis, another strain containing VanM/N genes... people are being quite kind to us! We seed the bacteria and transform them with the BioBricks, depending on the format they come in.
Our mini-preps are ready at last after a hard day’s work, and we are able to leave some inoculums of DH5-a to prepare competent cells tomorrow.
21.30: Lab OFF.
Monday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Tuesday 19 July
Materials and chemicals keep appearing in big cardboard boxes full of styro-foam pieces (we're storing it all in the hopes of filling a bath next month and having some fun).
More good news: our transformed cultures have grown as expected, as have the B. subtilis strains. At the end of the day we make inoculums of them all so that they grow o/n and tomorrow we can freeze samples and do yet more mini-preps (we have only just started and we're already sick and tired of that kit...)
We need to discuss which assembling method we'll use with the BioBricks. It takes us some hours, and in the meantime, Andrés faces the personal challenge of making competent cells from the DH5-a inoculum with the new protocol. Tomorrow we'll see who has won the battle...
And Pedro isn't wasting any time at all, he's just found out these racks are stackable, like Lego. This discovery has changed his life, look how happy he is!
After deciding how we'll build each BioBrick, and counting all the ligations we need to do, we finally tackle the digestions with the different restriction enzymes. Mastermix kits this time. More protocols. GREAT.
Tuesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Wednesday 20 July
AT LAST our official mascot is here! Say hello to Mr. Gibbs! Rocío had him held captive in her house, but to make up for that, she's brought a WONDERFUL Disney princess clock to cheer our lab up! It gives the lab that homely touch. We have also brought a coffee machine - we need caffeine in industrial amounts to work here for so many hours a day.
More mini-preps and frozen samples. But we're also trying something new! Our first electrophoresis to see how the digestions and the mini-preps go. We're examining the results. It's ok for the first time, but there's a long way to go yet...
Marta is in charge of our first ligations, tomorrow we'll see how they went.
...and how did the great battle end? Andrés 1 - E.coli 0. We have competent cells!
Out of the lab before 19.00. What a record!
Out of the lab before 19.00. What a record!
Wednesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Thursday 21 July
We run an electrophoresis of the digestions we made yesterday, but it hasn't worked properly, it seems the process still needs some fine-tuning.
We spend some time preparing the rewards for those who gave us money for the project through the crowd-funding platform. Letters, funny badges, videos...
Coffee break while we run one more electrophoresis...
We have a nice visit today! Lauren, from the team of Pennsylvania, is here to tell us about her project, and learn a bit more about ours. Carlos is having an intense discussion on kinetics with her while Marta struggles to design the primers we'll need for the PCR reactions next week. There's no way to get them exactly right, what a nightmare!
Thursday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Friday 22 July
It seems nothing's gone right so far except for the transformations. We'll have to start over...BUT WE HAVEN'T SAID OUR LAST WORD YET! [To be continued...]
Friday Protocols
Weekend
Blondie and little Rosidito didn't appear in the lab these two days so our bacterial cultures died and we have to lose time on Monday making new bacterial cultures. The rest of the group are starting to hate both clumsy girls. [Just kidding, we love them: they're the best, efficient and the most beautiful]
Weekend Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
