Revision as of 15:54, 20 July 2011

Transformation

Back to protocols.

Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: 2010 protocol

The protocol specifies to ask Henrike for plasmid quantities. Here is her answer:
- For relatively pure plasmids, 1 ul is enough.
- For low concentration plasmids, like those originating from a Gibson assembly, use 2 to 3 ul.

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 {{:Team:EPF-Lausanne/Templates/ProtocolHeader|title=Transformation}}
-Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf | 2010 protocol]
+Transformation is the introduction of a foreign plasmid into competent cells. We follow the protocol from last year's team: [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf 2010 protocol]
 == Additional notes ==