Team:Cornell/Week 5

From 2011.igem.org

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==Saturday==
==Saturday==
*GFP PCR product Gel Purified, Digested, PCR clean up
*GFP PCR product Gel Purified, Digested, PCR clean up
-
        **pZE-12 Backbone plasmid was miniprepped, digested, Gel Purified
+
**pZE-12 Backbone plasmid was miniprepped, digested, Gel Purified
-
        **Primer pair A and B were phosphorylated and annealed
+
**Primer pair A and B were phosphorylated and annealed
-
          **Ligation of all three components done overnight.  
+
**Ligation of all three components done overnight.  
*Sent out sponsorship emails to NEB, life technologies, agilent, and synthetic Genomics.
*Sent out sponsorship emails to NEB, life technologies, agilent, and synthetic Genomics.
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__NOTOC__

Revision as of 03:35, 18 July 2011

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


July 3rd - July 9th

Sunday

Monday

Tuesday

Wednesday

  • Sent out sponsorship emails to Roche, Promega, and Fermentas

Thursday

  • Put together CSS template for wiki homepage and content pages, as well as starting to make graphics

Friday

  • GFP PCR- ed off backbone,
    • Primer pair A and B were phosphorylated and annealed

Saturday

  • GFP PCR product Gel Purified, Digested, PCR clean up
    • pZE-12 Backbone plasmid was miniprepped, digested, Gel Purified
    • Primer pair A and B were phosphorylated and annealed
    • Ligation of all three components done overnight.
  • Sent out sponsorship emails to NEB, life technologies, agilent, and synthetic Genomics.