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Team:Edinburgh
From 2011.igem.org
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Welcome to '''Edinburgh''''s 2011 iGEM effort, a.k.a. '''Team Synergy'''. | Welcome to '''Edinburgh''''s 2011 iGEM effort, a.k.a. '''Team Synergy'''. | ||
| - | + | This year we will create microscopic '''"bioreactors"''', consisting of scaffolds for various enzymes, to carry out reactions in an extracellular environment. The hope is that, by combining the activity of multiple '''synergistic''' enzymes in a small space, high efficiency will be achieved. At least 4 systems are being considered: | |
| + | |||
| + | * As a baseline, use '''bacteria''' as the scaffold, and attach enzymes by cell-surface display techniques. | ||
| + | |||
| + | * As a modified version of the above, use bacteria, but concentrate the enzymes on a small part of it such as the '''flagella'''. | ||
| + | |||
| + | * As a fairly novel concept, use '''M13 phage''' as the scaffold, and attach enzymes by phage-display techniques to the pVIII coat protein. | ||
| + | |||
| + | * As a modified version of the above, attach multiple such phage to '''beads''' via the pIII protein, making a larger "reactor". | ||
| + | |||
| + | As example systems, we will (probably!) use '''cellulases''' as our enzymes of interest. | ||
| + | |||
| + | Additionally, it would be good if we could also create something from the sugar we hopefully generate. This would involve creation of a '''biorefinery'''. | ||
Revision as of 14:51, 15 July 2011
Welcome to Edinburgh's 2011 iGEM effort, a.k.a. Team Synergy.
This year we will create microscopic "bioreactors", consisting of scaffolds for various enzymes, to carry out reactions in an extracellular environment. The hope is that, by combining the activity of multiple synergistic enzymes in a small space, high efficiency will be achieved. At least 4 systems are being considered:
- As a baseline, use bacteria as the scaffold, and attach enzymes by cell-surface display techniques.
- As a modified version of the above, use bacteria, but concentrate the enzymes on a small part of it such as the flagella.
- As a fairly novel concept, use M13 phage as the scaffold, and attach enzymes by phage-display techniques to the pVIII coat protein.
- As a modified version of the above, attach multiple such phage to beads via the pIII protein, making a larger "reactor".
As example systems, we will (probably!) use cellulases as our enzymes of interest.
Additionally, it would be good if we could also create something from the sugar we hopefully generate. This would involve creation of a biorefinery.
