"

From 2011.igem.org

Revision as of 13:51, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→3A-assembly: Ligation and transformation) ← Older edit		Revision as of 13:51, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→PCR) Newer edit →
Line 59:		Line 59:
	DNA template:		DNA template:
	*BBa_I150010		*BBa_I150010
		+	<br/>
		+	<br/>
	==<span style="color:orange;">Lysis cassette</span>==		==<span style="color:orange;">Lysis cassette</span>==

---

Revision as of 13:51, 15 July 2011

Contents 1 green light receptor 1.1 NAME OF YOUR EXPERIMENT 2 blue light receptor 2.1 NAME OF YOUR EXPERIMENT 3 red light receptor 3.1 3A-assembly: Digest 3.2 3A-assembly: Ligation and transformation 3.3 PCR 4 Lysis cassette 4.1 NAME OF YOUR EXPERIMENT 5 Precipitator 5.1 Test-digest

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

3A-assembly: Digest

Investigators: Jakob

3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)

S21a:

• Enzyme 1: XbaI
• Enzyme 2: PstI

S22, S23

• Enzyme 1: EcoRI
• Enzyme 2: SpeI

Vector:

• Enzyme 1: EcoRI
• Enzyme 2: PstI
• Enzyme 3: DpnI

3A-assembly: Ligation and transformation

Investigators: Julia

Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.

L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3

Plasmids were transformed in cells and plated out.

PCR

Investigators: Julia

PCR of the red light receptor, cph8.

Primer:

• P 10
• P 11

DNA template:

• BBa_I150010

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

Test-digest

Investigators: Rüdiger

Testdigest of the minipreps, Sophie did (june, 13).

• Enzyme 1: PstI
• ENzyme 2: EcoRI

Image of different samples from miniprep.