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Revision as of 12:13, 14 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→13. Labday) ← Older edit		Revision as of 09:44, 15 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→3A assembly: Ligation) Newer edit →
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-	==3A assembly: Ligation==	+	==<span style="color:orange;">Lysis cassette</span>==
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	'''Investigators: Theo'''		'''Investigators: Theo'''

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Revision as of 09:44, 15 July 2011

Lysis cassette

3A assembly: Ligation

Investigators: Theo

Comments: Ligation of digested parts from yesterday.

S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector

Transformation

Investigators: Theo

Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.