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Team:EPF-Lausanne/Protocols/Liquid cultures
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Latest revision as of 08:48, 15 July 2011
Suspension cell cultures
Back to protocols.As we are dealing with bacteria, work under a flame!
First, prepare the medium in a falcon tube. One tube per colonie you will pick:
- 5 ml LB medium (be careful not to touch the walls of the bottle!)
- 5ul ampicillin (or other antibiotic)
Then, for each colonie, drop a tip in it (don't make a hole in the agar) and put the tip in the tube. The tubes should be incubated overnight at 37°C; put the tubes 30°C inclined.
