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Revision as of 08:12, 13 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→3A assembly: Ligation) ← Older edit		Revision as of 12:13, 14 July 2011 (view source) Sandra.Wassner (Talk | contribs) (→13. Labday) Newer edit →
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	==3A assembly: Ligation==		==3A assembly: Ligation==

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Revision as of 12:13, 14 July 2011

3A assembly: Ligation

Investigators: Theo

Comments: Ligation of digested parts from yesterday.

S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector

Transformation

Investigators: Theo

Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.