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Team:EPF-Lausanne/Protocols/Glycerol stock
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Revision as of 08:25, 14 July 2011
Glycerol stock
Material needed: overnight cultures of transformed cells (do not centrifuge them) and glycerol 50%
- Mix gently the cell cultures to resuspend the pellet
- In an Effendorf tube, put 500 ul of glycerol 50% and 500 ul of cell culture (always keep 1:1 ratio)
- Label the tube
- Mix gently and put the Eppendrofs quickly in the -80°C freezer (glycerol is toxic for the cells)
