Team:Northwestern/Notebook/Protocols/Overnight

From 2011.igem.org

(Difference between revisions)
Helenmelon (Talk | contribs)
(Created page with "__NOTOC__ {{:Team:Northwestern/Templates/trial}} <DIV style="font-size:20px"> Overnight</DIV> ---------------------- '''REMEMBER TO USE PROPER STERILE TECHNIQUE''' *Take 5mL ...")
Newer edit →

Revision as of 20:42, 11 July 2011

RETURN TO IGEM 2010

Overnight

REMEMBER TO USE PROPER STERILE TECHNIQUE


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube 
  *MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER

[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware

Back to Protocol

Retrieved from "http://2011.igem.org/Team:Northwestern/Notebook/Protocols/Overnight"