Team:NTNU Trondheim/Biobricks

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=Team NTNU's BioBricks=
=Team NTNU's BioBricks=
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{|
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|
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|
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|NTNU's BioBricks
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|----
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|Short
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|Name
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|Part number
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|Plasmid
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|Resistance
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|Length
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|Comment
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|Position
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|Link to part
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|----
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|P1
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|rrnB P1
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|BBa_K112118
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|BBb1 entry vector
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|Spec
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|503
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|Contains the rrnB P1 promoter, possibly also junk
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|Plate 3, 15L
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|----
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|lambdaPR
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|lambda pR mod
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|BBa_R0051
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|pSB1A2
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|Amp
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|49
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|Strong promoter, based on lambda pR, repressible by ci, possibly ppGpp
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|Plate 1, 6K
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|----
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|RFP
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|TetR + p(TetR)+RFP
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|BBa_K092600
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|pSB1A2
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|Amp
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|1634
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|RFP inhibited by tetR, RFP produced by neg. Promoter on TetR
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|Plate 2, 22L
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|http://partsregistry.org/wiki/index.php/Part:BBa_K092600
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|----
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|Lux
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|TetR + p(TetR)+RFP+luxI
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|BBa_K092700
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|pSB1A2
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|Amp
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|2365
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|Same as BBa_K092600, but with luxI in addition
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|Plate 2, 22N
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|----
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|lacP
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|lac Promoter (lacI regulated, lambda pL hybrid)
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|BBa_R0011
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|pSB1A2
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|Amp
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|55
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|Strong promoter, will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI
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|Plate 1, 6G
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|http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011
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|----
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|lacI
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|lacI repressor from E. coli (+LVA)
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|BBa_C0012
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|pSB1A2
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|Amp
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|1128
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|Coding region for the LacI protein with an LVA degradation tail and without an RBS. The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG
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|Plate 1, 2O
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|http://partsregistry.org/wiki/index.php/Part:BBa_C0012
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|----
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|mC
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|mCherry, bacterial with RBS and forward terminator
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|BBa_J06702
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|pSB1A2
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|Amp (AK/A)
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|869
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|mCherry, bacterial with RBS and forward terminator
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|Plate 2, 8E
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|http://partsregistry.org/partsdb/get_part.cgi?part=BBa_J06702
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|----
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|lacI
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|lacI repressor with RBS
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|BBa_J24679
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|pSB1A2
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|Amp
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|1170
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|RBS added to LacI protein coding region
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|Plate 1, 7L
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|http://partsregistry.org/partsdb/get_part.cgi?part=BBa_J24679
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|----
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|TERM
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|Double terminator
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|BBa_B0015
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|pSB1AK3
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|Amp (AK)
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|129
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|Dobbel terminator, mest brukt
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|Plate 1, 23L
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|http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0015
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|----
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|
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|Strong RBS + LacI(LVAtag) + double terminator
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|BBa_K292006
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|pSB1A2 ?
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|
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|1277
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|This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005.
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|Ikke i distribution, bestilt 29/6
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|http://partsregistry.org/Part:BBa_K292006
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|----
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|GFP
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|GFP(+LVA)
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|BBa_K082003
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|pSB1AK3
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|AK/AK
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|756
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|GFP with degradation LVA tag
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|Plate 4, 12I
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|http://partsregistry.org/wiki/index.php/Part:BBa_K082003
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|----
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|RBS
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|Ribosome Binding Site (strong)
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|BBa_B0034
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|pSB1A2
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|Amp
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|12
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|Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. Brukes i masse forskjellige constructs
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|Plate 1, 2M
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|http://partsregistry.org/wiki/index.php/Part:BBa_B0034
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|----
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|TERM2
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|Double terminator
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|BBa_B0014
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|pSB1AK3
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|AK/AK
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|
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|
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|Plate 2, 24C
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|http://partsregistry.org/Part:BBa_B0014
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|----
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|pBAD
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|Inducible pBad/araC promoter
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|BBa_I0500
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|pSB2K3
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|K
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|1210
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|pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. repressor: AraC apparently acts as the repressor
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|Plate 1, 14N, Plate 3 20B, Plate 4 16K
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|http://partsregistry.org/Part:BBa_I0500
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|----
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|pBAD2
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|Alternative  pBAD/araC
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|BBa_K113009
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|pSB1A2
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|A
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|1210
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|Says BBa_I0050 doesn't work, made new pBAD/AraC
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|Plate 3, 1M
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|http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K113009
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|----
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|}
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Revision as of 17:10, 11 July 2011



Team NTNU's BioBricks

NTNU's BioBricks
Short Name Part number Plasmid Resistance Length Comment Position Link to part
P1 rrnB P1 BBa_K112118 BBb1 entry vector Spec 503 Contains the rrnB P1 promoter, possibly also junk Plate 3, 15L
lambdaPR lambda pR mod BBa_R0051 pSB1A2 Amp 49 Strong promoter, based on lambda pR, repressible by ci, possibly ppGpp Plate 1, 6K
RFP TetR + p(TetR)+RFP BBa_K092600 pSB1A2 Amp 1634 RFP inhibited by tetR, RFP produced by neg. Promoter on TetR Plate 2, 22L http://partsregistry.org/wiki/index.php/Part:BBa_K092600
Lux TetR + p(TetR)+RFP+luxI BBa_K092700 pSB1A2 Amp 2365 Same as BBa_K092600, but with luxI in addition Plate 2, 22N
lacP lac Promoter (lacI regulated, lambda pL hybrid) BBa_R0011 pSB1A2 Amp 55 Strong promoter, will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI Plate 1, 6G http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011
lacI lacI repressor from E. coli (+LVA) BBa_C0012 pSB1A2 Amp 1128 Coding region for the LacI protein with an LVA degradation tail and without an RBS. The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG Plate 1, 2O http://partsregistry.org/wiki/index.php/Part:BBa_C0012
mC mCherry, bacterial with RBS and forward terminator BBa_J06702 pSB1A2 Amp (AK/A) 869 mCherry, bacterial with RBS and forward terminator Plate 2, 8E http://partsregistry.org/partsdb/get_part.cgi?part=BBa_J06702
lacI lacI repressor with RBS BBa_J24679 pSB1A2 Amp 1170 RBS added to LacI protein coding region Plate 1, 7L http://partsregistry.org/partsdb/get_part.cgi?part=BBa_J24679
TERM Double terminator BBa_B0015 pSB1AK3 Amp (AK) 129 Dobbel terminator, mest brukt Plate 1, 23L http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0015
Strong RBS + LacI(LVAtag) + double terminator BBa_K292006 pSB1A2 ? 1277 This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. Ikke i distribution, bestilt 29/6 http://partsregistry.org/Part:BBa_K292006
GFP GFP(+LVA) BBa_K082003 pSB1AK3 AK/AK 756 GFP with degradation LVA tag Plate 4, 12I http://partsregistry.org/wiki/index.php/Part:BBa_K082003
RBS Ribosome Binding Site (strong) BBa_B0034 pSB1A2 Amp 12 Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. Brukes i masse forskjellige constructs Plate 1, 2M http://partsregistry.org/wiki/index.php/Part:BBa_B0034
TERM2 Double terminator BBa_B0014 pSB1AK3 AK/AK Plate 2, 24C http://partsregistry.org/Part:BBa_B0014
pBAD Inducible pBad/araC promoter BBa_I0500 pSB2K3 K 1210 pBad is an E. coli promoter that is tightly controlled by:inducer: L-arabinose. repressor: AraC apparently acts as the repressor Plate 1, 14N, Plate 3 20B, Plate 4 16K http://partsregistry.org/Part:BBa_I0500
pBAD2 Alternative pBAD/araC BBa_K113009 pSB1A2 A 1210 Says BBa_I0050 doesn't work, made new pBAD/AraC Plate 3, 1M http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K113009


BioBrick no. Name Description Comment




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