Team:EPF-Lausanne/Protocols/TetR

From 2011.igem.org

(Difference between revisions)
(PCR Cycle (30 cycles))
(PCR Cycle (10 cycles))
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== PCR Cycle (10 cycles) ==
== PCR Cycle (10 cycles) ==
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<b>1.</b> 98°C for 30s
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:<b>1.</b> 98°C for 30s
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<b>2.</b> 98°C for 7.5 seconds
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:<b>2.</b> 98°C for 7.5 seconds
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<b>3.</b> 58°C for 20 sec  
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:<b>3.</b> 58°C for 20 sec  
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<b>4.</b> 72°C for 15 sec;  
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:<b>4.</b> 72°C for 15 sec;  
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<b>5.</b> 72°C for 5 min
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:<b>5.</b> 72°C for 5 min
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<b>6.</b> 4°C "forever"
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:<b>6.</b> 4°C "forever"
== Materials ==
== Materials ==

Revision as of 10:57, 10 July 2011