Team:EPF-Lausanne/Notebook/June2011

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(Gradient PCR)
(Gradient PCR)
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In a last attempt to try to amplify the P23019 plasmid backbone (the last piece needed to start a Gibson assembly of the TetR plasmid), Vincent tried a gradient PCR in which separate samples of the plasmid would be run at temperatures between 40 and 50 C (40, 42, 44, 46, and 48). The results showed, once again, that amplification was beyond reach.  
In a last attempt to try to amplify the P23019 plasmid backbone (the last piece needed to start a Gibson assembly of the TetR plasmid), Vincent tried a gradient PCR in which separate samples of the plasmid would be run at temperatures between 40 and 50 C (40, 42, 44, 46, and 48). The results showed, once again, that amplification was beyond reach.  
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[[File:EPFL-29-06-p23019fail.jpg]]
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[[File:EPFL-29-06-p23019fail.jpg|600px|GFP-tetR and cy5 fluorescence]]
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Revision as of 17:25, 8 July 2011