Team:Freiburg/Notebook/8 June
From 2011.igem.org
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(→Amplification of different parts) |
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|P1, 5A | |P1, 5A | ||
|kan | |kan | ||
- | | | + | |high copy BioBrick assemby plasmid |
|- | |- | ||
|pSB1A3 | |pSB1A3 | ||
|P1, 1G | |P1, 1G | ||
|amp | |amp | ||
- | | | + | |high copy BioBrick assemby plasmid |
|- | |- | ||
|pSB1C3 | |pSB1C3 | ||
|P1,3A | |P1,3A | ||
|cm | |cm | ||
- | | | + | |high copy BioBrick assemby plasmid |
|- | |- | ||
|pSB1T3 | |pSB1T3 | ||
|P1, 7A | |P1, 7A | ||
|tet | |tet | ||
- | | | + | |high copy BioBrick assemby plasmid |
|- | |- | ||
|J23104 | |J23104 | ||
|P1, 18K | |P1, 18K | ||
| | | | ||
- | | | + | |promotor |
|- | |- | ||
|J23110 | |J23110 | ||
|P1, 20 C | |P1, 20 C | ||
| | | | ||
- | | | + | |promotor |
|- | |- | ||
|J23116 | |J23116 | ||
|P1, 20M | |P1, 20M | ||
| | | | ||
- | | | + | |promotor |
|- | |- | ||
|B0034 | |B0034 | ||
|P1, 2M | |P1, 2M | ||
| | | | ||
- | | | + | |RBS (strong) |
|- | |- | ||
|B0032 | |B0032 | ||
|P1, 2 I | |P1, 2 I | ||
| | | | ||
- | | | + | |RBS.3 (medium) |
|- | |- | ||
|B0031 | |B0031 | ||
|P1, 2G | |P1, 2G | ||
| | | | ||
- | | | + | |RBS.2 (weak) |
|} | |} |
Revision as of 11:38, 8 July 2011
??.Labday
Protein modelling with pymol
Investigators: Rüdiger
Amplification of different parts
Investigators: Julia and Jacob
antibiotics plates:
1oo mg/ml ampicillin (amp) (100 microliter per plate)
1oo mikroliter H2O + 2 microliter canamycin (cm)
1oo mikroliter H2O + 40 microliter tetracyclin(tet)
1oo mikroliter H2O + 10 microliter kanamycin (kn)
part | location(p=plate) | resistance | info |
BBa_K098995 | P3, 1E | amp | heat sensitive cI QPI with high promoter |
BBa_K112022 | P3, 24E | amp | Lambda phage lysis device - no promoter, This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. |
pSB1K3 | P1, 5A | kan | high copy BioBrick assemby plasmid |
pSB1A3 | P1, 1G | amp | high copy BioBrick assemby plasmid |
pSB1C3 | P1,3A | cm | high copy BioBrick assemby plasmid |
pSB1T3 | P1, 7A | tet | high copy BioBrick assemby plasmid |
J23104 | P1, 18K | promotor | |
J23110 | P1, 20 C | promotor | |
J23116 | P1, 20M | promotor | |
B0034 | P1, 2M | RBS (strong) | |
B0032 | P1, 2 I | RBS.3 (medium) | |
B0031 | P1, 2G | RBS.2 (weak) |