Team:Freiburg/Notebook/8 June

From 2011.igem.org

(Difference between revisions)
(Protein modelling and amplification of parts)
(Protein modelling and amplification of parts)
Line 8: Line 8:
1oo mikroliter H2O + 40 microliter Tet<br/>
1oo mikroliter H2O + 40 microliter Tet<br/>
1oo mikroliter H2O + 10 microliter Kan<br/>
1oo mikroliter H2O + 10 microliter Kan<br/>
 +
 +
{| border="1" align="center" style="text-align:center;"
 +
|'''part'''
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|'''location(p=plate)'''
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|'''resistance'''
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|'''info'''
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|-
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|BBa_K098995
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|P3, 1E
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|Amp
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|heat sensitive cI QPI with high promoter
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|-
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|BBa_K112022
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|P3, 24E
 +
|amp
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|Lambda phage lysis device - no promoter, This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI.
 +
|}

Revision as of 10:37, 8 July 2011

Protein modelling and amplification of parts

Investigators:
Protein modelling with pymol: Rüdiger
Amplification of different parts: Julia and Jacob

antibiotics plates:
1oo mg/ml Ampizilin (100 microliter per plate)
1oo mikroliter H2O + 2 microliter Cm
1oo mikroliter H2O + 40 microliter Tet
1oo mikroliter H2O + 10 microliter Kan

part location(p=plate) resistance info
BBa_K098995 P3, 1E Amp heat sensitive cI QPI with high promoter
BBa_K112022 P3, 24E amp Lambda phage lysis device - no promoter, This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI.