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Team:Paris Bettencourt/Experiment/T7 diff subt subt microfluidic
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<h1>Nanotube-assisted diffusion of T7 polymerase in Microfluidics</h1> | <h1>Nanotube-assisted diffusion of T7 polymerase in Microfluidics</h1> | ||
| - | <h2>Experimental Scheme<h2> | + | <h2>Experimental Scheme</h2> |
<p>In order to test whether our <i>Bacillus subtilis</i> T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent <a href="http://biodynamics.ucsd.edu/pubs/articles/Mondragon11.pdf">paper</a> <a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ#references">[1]</a>. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase | <p>In order to test whether our <i>Bacillus subtilis</i> T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent <a href="http://biodynamics.ucsd.edu/pubs/articles/Mondragon11.pdf">paper</a> <a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ#references">[1]</a>. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase | ||
Revision as of 02:10, 29 October 2011
Nanotube-assisted diffusion of T7 polymerase in Microfluidics
Experimental Scheme
In order to test whether our Bacillus subtilis T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent paper [1]. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase
References
- Entrainment of a population of synthetic genetic oscillators. Mondragón-Palomino, O., Danino, T., Selimkhanov, J., Tsimring, L. & Hasty, J. Science 333, 1315-1319 (2011).
