Team:UTP-Panama/After Regional Week 1

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(→Octuber 17 to 23)
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== Octuber 17 to 23==
+
== October 17 to 23==
-
.
+
 
 +
== October 18th ==
 +
INVENTORY:
 +
 
 +
{|border="1" style="text-align:center;"
 +
|Quantity
 +
|Description
 +
|-
 +
|24
 +
|petri plates (60 x 15 mm)
 +
|-
 +
|a lot!
 +
|eppendorf tubes of 0,5 mL
 +
|-
 +
|4
 +
|tubes with linearized plasmids (kit)
 +
|-
 +
|8
 +
|tubes with competent cells
 +
|-
 +
| -
 +
|genetic material from minipreparations
 +
|-
 +
|?
 +
|ligation kit
 +
|-
 +
|2
 +
|tubes with SOC media (15 mL)
 +
|-
 +
|2
 +
|tubes with cloramphenicol (liquid or powder)
 +
|-
 +
|2
 +
|petri dishes with GaTech colonies
 +
|-
 +
|1
 +
|petri plates with RFP colonies
 +
|-
 +
|1 jar
 +
|MgCl2 (for doing comp. cells)
 +
|-
 +
|1 jar
 +
|CaCl2 (for doing comp. cells)
 +
|-
 +
|1 jar
 +
|with ultrapure water
 +
|-
 +
|3 jars
 +
|minipreparation Buffers (P1,P2,P3)
 +
|-
 +
|3 boxes
 +
|small pipette tips (10 uL)
 +
|-
 +
|4 boxes
 +
|medium pipette tips (200 uL)
 +
|-
 +
|3 boxes
 +
|large pipette tips (1000 uL)
 +
|-
 +
|35
 +
|falcon tubes of 15 mL
 +
|}
 +
 
 +
Missing:
 +
<br>- large gloves
 +
<br>- falcon tubes 50 mL
 +
<br>- antibiotics
 +
<br>- LB media
 +
<br>- Eppendorf 2 mL for auto-nailing
 +
<br><br>
 +
 
 +
== October 19th ==
 +
Ricardo reviewed  the inventory and provided us:
 +
<br>- 1 box of large gloves
 +
<br>- 1 bag with falcons 50 mL
 +
<br>- 1 bag with eppendorf of 2 mL
 +
<br><br>
 +
Also:
 +
<br>- solid LB works
 +
<br>- not necessary to do liquid LB (there is)
 +
<br>- we have to transform competent cells with:
 +
<br>  - Bristol (the plate isn't so good)
 +
<br>  - RENBO (the product was ligated in the ampicillin plasmid) --> to do minipreparation and electrophoresis
 +
<br>  - Colonies only without plasmids.
 +
<br><br> Plates with RFP and GaTech are fine!
 +
<br><br>
 +
We had to:
 +
<br>- prepare new antibiotics
 +
<br>- auto-nail the eppendorf of 2 mL, they were ready.
 +
<br>- prepare the Minimal Media for the experiments.

Revision as of 23:29, 28 October 2011

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October 17 to 23

October 18th

INVENTORY:

Quantity Description
24 petri plates (60 x 15 mm)
a lot! eppendorf tubes of 0,5 mL
4 tubes with linearized plasmids (kit)
8 tubes with competent cells
- genetic material from minipreparations
? ligation kit
2 tubes with SOC media (15 mL)
2 tubes with cloramphenicol (liquid or powder)
2 petri dishes with GaTech colonies
1 petri plates with RFP colonies
1 jar MgCl2 (for doing comp. cells)
1 jar CaCl2 (for doing comp. cells)
1 jar with ultrapure water
3 jars minipreparation Buffers (P1,P2,P3)
3 boxes small pipette tips (10 uL)
4 boxes medium pipette tips (200 uL)
3 boxes large pipette tips (1000 uL)
35 falcon tubes of 15 mL

Missing:
- large gloves
- falcon tubes 50 mL
- antibiotics
- LB media
- Eppendorf 2 mL for auto-nailing

October 19th

Ricardo reviewed the inventory and provided us:
- 1 box of large gloves
- 1 bag with falcons 50 mL
- 1 bag with eppendorf of 2 mL

Also:
- solid LB works
- not necessary to do liquid LB (there is)
- we have to transform competent cells with:
- Bristol (the plate isn't so good)
- RENBO (the product was ligated in the ampicillin plasmid) --> to do minipreparation and electrophoresis
- Colonies only without plasmids.

Plates with RFP and GaTech are fine!

We had to:
- prepare new antibiotics
- auto-nail the eppendorf of 2 mL, they were ready.


- prepare the Minimal Media for the experiments.
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