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Team:Harvard/Human Practices/Timeline
From 2011.igem.org
(Difference between revisions)
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<li><a href="#">2011</a></li> | <li><a href="#">2011</a></li> | ||
| - | <li><a href="#">Summer | + | <li><a href="#">Summer</a></li> |
</ul> | </ul> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/1.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/1.png" width="256" height="256" /> | ||
| - | < | + | <h2>Discovery of the zinc finger protein</h2> |
<p>Sir Aaron Klug first identifies the repeated binding motif in Transcription Factor IIIA and is the first to use the term ‘zinc finger’.</p> | <p>Sir Aaron Klug first identifies the repeated binding motif in Transcription Factor IIIA and is the first to use the term ‘zinc finger’.</p> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/2.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/2.png" width="256" height="256" /> | ||
| - | < | + | <h2>First zinc finger crystal structure</h2> |
<p>Carl Pabo and Nikola Pavletich of Johns Hopkins University solve the crystal structure of zif268, now the most-commonly studied zinc finger.</p> | <p>Carl Pabo and Nikola Pavletich of Johns Hopkins University solve the crystal structure of zif268, now the most-commonly studied zinc finger.</p> | ||
| Line 110: | Line 110: | ||
<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/3.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/3.png" width="256" height="256" /> | ||
| - | < | + | <h2>CEO Edward Lanphier founds Sangamo Biosciences</h2> |
<p>Edward Lanphier leaves Somatix Therapy Corporation and makes a deal for exclusive rights to the work of Srinivan Chandrasegaran of Johns Hopkins University who combined the Fok I nuclease with zinc fingers.</p> | <p>Edward Lanphier leaves Somatix Therapy Corporation and makes a deal for exclusive rights to the work of Srinivan Chandrasegaran of Johns Hopkins University who combined the Fok I nuclease with zinc fingers.</p> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/4.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/4.png" width="256" height="256" /> | ||
| - | < | + | <h2>Fok I nuclease successfully fused to zinc fingers</h2> |
<p>Attaching nuclease proteins to zinc fingers opened up possibilities for future research in gene therapy by allowing researchers to directly modify the genome though nuclease cutting.</p> | <p>Attaching nuclease proteins to zinc fingers opened up possibilities for future research in gene therapy by allowing researchers to directly modify the genome though nuclease cutting.</p> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/5.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/5.png" width="256" height="256" /> | ||
| - | < | + | <h2>Sangamo enters the public sector</h2> |
<p></p> | <p></p> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/6.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/6.png" width="256" height="256" /> | ||
| - | < | + | <h2>Sangamo patents zinc finger nuclease technology"</h2> |
<p>Any use or production of zinc fingers with attached nucleases is the intellectual property of Sangamo.</p> | <p>Any use or production of zinc fingers with attached nucleases is the intellectual property of Sangamo.</p> | ||
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<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/7.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/7.png" width="256" height="256" /> | ||
| - | < | + | <h2>Zinc finger nucleases become open source</h2> |
<p>Researcher Keith Joung of Harvard University and Mass. General Hospital develops a method for making zinc finger nuclease proteins that bind to custom target sequences.</p> | <p>Researcher Keith Joung of Harvard University and Mass. General Hospital develops a method for making zinc finger nuclease proteins that bind to custom target sequences.</p> | ||
| Line 160: | Line 160: | ||
<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/8.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/8.png" width="256" height="256" /> | ||
| - | < | + | <h2>Sangamo and Sigma-Aldrich sign licensing deal</h2> |
<p>The license allows Sigma-Aldrich to sell custom zinc finger nuclease protein pairs for approximately $30,000 each.</p> | <p>The license allows Sigma-Aldrich to sell custom zinc finger nuclease protein pairs for approximately $30,000 each.</p> | ||
| Line 170: | Line 170: | ||
<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/9.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/9.png" width="256" height="256" /> | ||
| - | < | + | <h2>Context-dependency improves open-source zinc finger engineering</h2> |
<p>Keith Joung publishes tables of zinc finger binding sites that account for context-dependent effects and can be rearranged to form custom zinc finger proteins that bind to a variety of DNA sequences.</p> | <p>Keith Joung publishes tables of zinc finger binding sites that account for context-dependent effects and can be rearranged to form custom zinc finger proteins that bind to a variety of DNA sequences.</p> | ||
| Line 176: | Line 176: | ||
</li> | </li> | ||
| - | <li id="#Summer | + | <li id="#Summer"> |
<img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/10.png" width="256" height="256" /> | <img src="http://people.fas.harvard.edu/~jwchew/iGEM/images/10.png" width="256" height="256" /> | ||
| - | < | + | <h2>Harvard iGEM develops a novel method to engineer custom zinc fingers</h2> |
| - | <p> | + | <p>Harvard iGEM uses novel integration of existing technologies for rapid, massive production of custom zinc fingersWe have developed a rapid, comparatively low-cost, <em>open source</em> method for making thousands of custom zinc fingers by integrating MAGE, lambda red, and chip-based synthesis technologies.</p> |
</li> | </li> | ||
Revision as of 23:05, 28 October 2011
Zinc Finger Historical Timeline
-
Discovery of the zinc finger protein
Sir Aaron Klug first identifies the repeated binding motif in Transcription Factor IIIA and is the first to use the term ‘zinc finger’.
-
First zinc finger crystal structure
Carl Pabo and Nikola Pavletich of Johns Hopkins University solve the crystal structure of zif268, now the most-commonly studied zinc finger.
-
CEO Edward Lanphier founds Sangamo Biosciences
Edward Lanphier leaves Somatix Therapy Corporation and makes a deal for exclusive rights to the work of Srinivan Chandrasegaran of Johns Hopkins University who combined the Fok I nuclease with zinc fingers.
-
Fok I nuclease successfully fused to zinc fingers
Attaching nuclease proteins to zinc fingers opened up possibilities for future research in gene therapy by allowing researchers to directly modify the genome though nuclease cutting.
-
Sangamo enters the public sector
-
Sangamo patents zinc finger nuclease technology"
Any use or production of zinc fingers with attached nucleases is the intellectual property of Sangamo.
-
Zinc finger nucleases become open source
Researcher Keith Joung of Harvard University and Mass. General Hospital develops a method for making zinc finger nuclease proteins that bind to custom target sequences.
-
Sangamo and Sigma-Aldrich sign licensing deal
The license allows Sigma-Aldrich to sell custom zinc finger nuclease protein pairs for approximately $30,000 each.
-
Context-dependency improves open-source zinc finger engineering
Keith Joung publishes tables of zinc finger binding sites that account for context-dependent effects and can be rearranged to form custom zinc finger proteins that bind to a variety of DNA sequences.
-
Harvard iGEM develops a novel method to engineer custom zinc fingers
Harvard iGEM uses novel integration of existing technologies for rapid, massive production of custom zinc fingersWe have developed a rapid, comparatively low-cost, open source method for making thousands of custom zinc fingers by integrating MAGE, lambda red, and chip-based synthesis technologies.
