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Team:Grenoble/Projet/Biobricks
From 2011.igem.org
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| - | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and | + | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composite parts we send to the registry.</li> |
</div class="blocbackground"> | </div class="blocbackground"> | ||
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<div class="blocbackground"> | <div class="blocbackground"> | ||
<ul> | <ul> | ||
| - | <li>You can also find in the registry some of our constructions | + | <li>You can also find in the registry some of our intermediate constructions :</li> |
</ul> | </ul> | ||
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<p align="left"> | <p align="left"> | ||
| - | <center><h5>MerR-del : (K545103)</h5></center><br/><br/> | + | <center><h5>MerR-del: (K545103)</h5></center><br/><br/> |
MerR (BBa_K346001) without RBS<br/> | MerR (BBa_K346001) without RBS<br/> | ||
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Well 1: Positive control (pSB4K5)<br/> | Well 1: Positive control (pSB4K5)<br/> | ||
Well 2: Negative control (Without DNA)<br/> | Well 2: Negative control (Without DNA)<br/> | ||
| - | Wells 3, 4, 5, 6, 7: MerR-del (expected at 740pb, | + | Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)<br/><br/> |
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
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Well 1: MW<br/> | Well 1: MW<br/> | ||
Well 2: empty well<br/> | Well 2: empty well<br/> | ||
| - | Well 3 and 4: | + | Well 3 and 4: clonning failure<br/> |
| - | Well 5: pMerT-GFP (expected at 1200pb | + | Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)<br/><br/> |
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
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PCR gel<br/><br/> | PCR gel<br/><br/> | ||
| - | Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, | + | Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)<br/> |
Well 4: MW<br/><br/> | Well 4: MW<br/><br/> | ||
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Wells 1 and 2: pConst-CinR(expected at 950pb)<br/> | Wells 1 and 2: pConst-CinR(expected at 950pb)<br/> | ||
| - | Wells 4 and 5: | + | Wells 4 and 5: cloning failure<br/> |
Wells 3 and 6: empty wells<br/> | Wells 3 and 6: empty wells<br/> | ||
| - | plasmids | + | plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/> |
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
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Well 1: pLac-CinR (expected at 950pb)<br/> | Well 1: pLac-CinR (expected at 950pb)<br/> | ||
Well 2: pConst-CinR (expected at 900pb)<br/> | Well 2: pConst-CinR (expected at 900pb)<br/> | ||
| - | plasmids | + | plasmids were obtained for each well at the expected size,about 2000pb.<br/><br/> |
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
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Well 1: RBS-CinR (expected at 1000)<br/> | Well 1: RBS-CinR (expected at 1000)<br/> | ||
| - | Wells 2, 3 and 4: | + | Wells 2, 3 and 4: clonning failure<br/><br/> |
Confirmed by sequencing<br/> | Confirmed by sequencing<br/> | ||
Revision as of 22:17, 28 October 2011
Our Biobricks:
- Here you can find all our news and composite parts we send to the registry.
<groupparts>iGEM011 Grenoble</groupparts>
- You can also find in the registry some of our intermediate constructions :
BBa_S04602 RBS-CinR, BBa_B0034+BBa_C0077
BBa_S04603 RBS-TetR, BBa_B0034+BBa_C0040
BBa_S04606 RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605 RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607 pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618 pLac-CinR, BBa_K545006+BBa_I13401
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MerR-del: (K545103)MerR (BBa_K346001) without RBS PCR gel Well 1: Positive control (pSB4K5) Well 2: Negative control (Without DNA) Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb) Confirmed by sequencing |
 |
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pMerT-GFP: (K545002)Original parts: K346002, E0240 PCR gel Well 1: MW Well 2: empty well Well 3 and 4: clonning failure Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb) Confirmed by sequencing |
 |
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pCin-CrtEBI (K545000)Original parts: R0077, K274100 PCR gel Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb) Well 4: MW Confirmed by sequencing |
 |
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pConst-RBS-CinR (K545004)Original parts: J23119, B0034 C0077 Restriction gel Wells 1 and 2: pConst-CinR(expected at 950pb) Wells 4 and 5: cloning failure Wells 3 and 6: empty wells plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing |
 |
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pLac-CinR (S04607)Original parts: R0011, E0240 Restriction gel pConst-GFP (K545001)Original parts: J23119, E0240 Restriction gel Well 1: pLac-CinR (expected at 950pb) Well 2: pConst-CinR (expected at 900pb) plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing |
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RBS-CinR (S04602)Original parts: B0034, C0077 PCR gel Well 1: RBS-CinR (expected at 1000) Wells 2, 3 and 4: clonning failure Confirmed by sequencing |
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RBS-TetR (S04603)Original parts: B0034, C0040 PCR gel well 1: RBS-TetR (expected at 950pb) Confirmed by sequencing |
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