"
Team:Paris Bettencourt/Experiments/YFP TetR diffusion experiments
From 2011.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
<p>We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from <i>E. coli</i> to <i>B. subtilis</i> by nanotube.<br> | <p>We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from <i>E. coli</i> to <i>B. subtilis</i> by nanotube.<br> | ||
</p> | </p> | ||
| + | |||
| + | <!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR --> | ||
| + | |||
| + | <div id="footer-wrapper"> | ||
| + | <div id="footer"> | ||
| + | <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list"> | ||
| + | |||
| + | |||
| + | <!-- Recentchanges is not handles well DEBUG --> | ||
| + | <li id="t-recentchanges"><a href="/Special:RecentChanges" | ||
| + | title='Recent changes'>Recent changes</a></li> | ||
| + | |||
| + | <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Paris_Bettencourt/Modeling" | ||
| + | title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li> | ||
| + | |||
| + | <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Paris_Bettencourt/Modeling" | ||
| + | title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li> | ||
| + | |||
| + | |||
| + | |||
| + | <li id="t-upload"><a href="/Special:Upload" | ||
| + | title="Upload files [u]" accesskey="u">Upload file</a> | ||
| + | </li> | ||
| + | <li id="t-specialpages"><a href="/Special:SpecialPages" | ||
| + | title="List of all special pages [q]" accesskey="q">Special pages</a> | ||
| + | |||
| + | </li> | ||
| + | <li><a href='/Special:Preferences'>My preferences</a></li> | ||
| + | </ul> | ||
| + | </div> <!-- close footer --> | ||
| + | <div id='footer'> | ||
| + | <ul id="f-list"> | ||
| + | |||
| + | <li id="t-print"><a href="/wiki/index.php?title=Team:Paris_Bettencourt/Modeling&printable=yes" | ||
| + | title="Printable version of this page [p]" accesskey="p">Printable version</a> | ||
| + | |||
| + | </li> | ||
| + | |||
| + | <li id="t-permalink"><a href="/wiki/index.php?title=Team:Paris_Bettencourt/Modeling&oldid=86565" | ||
| + | title="Permanent link to this revision of the page">Permanent link</a> | ||
| + | </li> | ||
| + | |||
| + | |||
| + | <li id="privacy"><a href="/2011.igem.org:Privacy_policy" title="2011.igem.org:Privacy policy">Privacy policy</a></li> | ||
| + | <li id="disclaimer"><a href="/2011.igem.org:General_disclaimer" title="2011.igem.org:General disclaimer">Disclaimers</a></li> | ||
| + | </ul> | ||
| + | |||
| + | </div> <!-- close footer --> | ||
| + | </div> <!-- close footer-wrapper --> | ||
| + | </div> | ||
| + | <div id="scroll_left"><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion"><img src="https://static.igem.org/mediawiki/2011/0/0a/Arrow-left-big.png" style="width:100%;"></a><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion">YFP concentrator design</a></div> | ||
| + | </html> | ||
Revision as of 19:44, 28 October 2011
YFP concentrator diffusion experiments
Design overview
YFP:TetR/TetO array system
More information on the design here.
Diffusion of YFP:tetR from E. coli to B. subtilis with TetO Array
We success to integrate the TetO Array in B. subtilis with the multihost episomal pHM3 plasmid.
Experiments of E. coli (pFX234 plasmid, D. Lane strains) to B. subtilis (TetO Array, pHM3) to follow an eventual fluorescence diffusion by nanotube has been tried.
Movie : Experiment of mixed YFP:tetR (E. coli) & TetO Array (B. subtilis)
We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from E. coli to B. subtilis by nanotube.
