Team:Grenoble/Projet/Biobricks

From 2011.igem.org

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       MerR-del : K545103.<br/>
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       <center><h5>MerR-del : K545103</h5></center><br/>
       MerR (BBa_K346001) without RBS<br/>
       MerR (BBa_K346001) without RBS<br/>
       PCR gel<br/>
       PCR gel<br/>
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     pMerT-GFP: K545002<br/>
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     <center><h5>pMerT-GFP: K545002</h5></center><br/><br/>
     Original parts: K346002, E0240<br/>
     Original parts: K346002, E0240<br/>
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     PCR gel<br/>
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     PCR gel<br/><br/>
     Well 1 and 2: Failed cloning<br/>
     Well 1 and 2: Failed cloning<br/>
     Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)<br/>
     Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)<br/>
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     pCin-CrtEBI (K545000)<br/>   
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     <center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>   
     Originally: R0077, K274100<br/>
     Originally: R0077, K274100<br/>
     PCR gel<br/>
     PCR gel<br/>
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     pConst-RBS-CinR (K545004)<br/>
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     <center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
     Originally J23119, B0034 C0077<br/>
     Originally J23119, B0034 C0077<br/>
     Restriction gel<br/>
     Restriction gel<br/>
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     pLac-CinR (S04607)<br/>
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     <center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
     Originally R0011, E0240<br/>
     Originally R0011, E0240<br/>
     pConst-GFP (K545001)<br/>
     pConst-GFP (K545001)<br/>
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     RBS-CinR (S04602)<br/>
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     <center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
     Originally B0034, C0077<br/>
     Originally B0034, C0077<br/>
     PCR gel<br/>
     PCR gel<br/>
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     RBS-TetR (S04603)<br/>
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     <center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
     Originally B0034, C0040<br/>
     Originally B0034, C0040<br/>
     PCR gel<br/>
     PCR gel<br/>

Revision as of 14:03, 27 October 2011

Grenoble 2011, Mercuro-Coli iGEM


Our Biobricks:

  • Here you can find all our news and composites parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

              		
			
  • You can also find in the registry some of our constructions intermediates:

BBa_S04602

RBS-CinR, BBa_B0034+BBa_C0077

BBa_S04603

RBS-TetR, BBa_B0034+BBa_C0040

BBa_S04606

RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077

BBa_S04605

RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240

BBa_S04607

pLac-CinR, BBa_R0011+BBa_C0077

BBa_S04618

pLac-CinR, BBa_K545006+BBa_I13401

MerR-del : K545103

MerR (BBa_K346001) without RBS
PCR gel
Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
Confirmed by sequencing

pMerT-GFP: K545002


Original parts: K346002, E0240
PCR gel

Well 1 and 2: Failed cloning
Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
Confirmed by sequencing

pCin-CrtEBI (K545000)


Originally: R0077, K274100
PCR gel
Expected at 3500pb, obtain between 3000 and 4000pb
Confirmed by sequencing

pConst-RBS-CinR (K545004)


Originally J23119, B0034 C0077
Restriction gel
Well 1, 2: failed cloning
Well 3, 4: pConst-CinR(expected at 950pb)
Confirmed by sequencing

pLac-CinR (S04607)


Originally R0011, E0240
pConst-GFP (K545001)
Originally J23119, E0240
Restriction gel
Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
Confirmed by sequencing

RBS-CinR (S04602)


Originally B0034, C0077
PCR gel
Well 1: RBS-CinR (expected at 1000)
Confirmed by sequencing

RBS-TetR (S04603)


Originally B0034, C0040
PCR gel
RBS-TetR (expected at 950pb)
Confirmed by sequencing