"
Team:Paris Bettencourt/Experiments/Methodologies/Integration
From 2011.igem.org
(Difference between revisions)
BaptisteCouly (Talk | contribs)
(Created page with "{{:Team:Paris_Bettencourt/tpl_test}} <h1>Integration plasmid</h1> In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis int...")
Newer edit →
(Created page with "{{:Team:Paris_Bettencourt/tpl_test}} <h1>Integration plasmid</h1> In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis int...")
Newer edit →
Revision as of 13:00, 26 October 2011
Integration plasmid
In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
 |
At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).
After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).
