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Team:Paris Bettencourt/Designs/List
From 2011.igem.org
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Revision as of 14:21, 19 October 2011
Design List
Concentrator
In order to observe a celar change in phenotype during diffusion experiments, we worked on a YFP-TetR fusion protein concentrator.
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YFP concentration This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
Positive feedback autoloop
We designed entirely these new devices. They are composed of an emitter, a receptor and an amplifier sub-unit.
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T7 RNA polymerase diffusion In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
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tRNA amber diffusion The tRNA amber allows the translation of a functionnal T7 RNA polymerase in the receiver cell. This will then trigger the auto-amplification loop. |
Using bistable switches
During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.
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ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
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Sin Operon |
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Lambda switch |
