Team:NYMU-Taipei/results/chassis1

From 2011.igem.org

(Difference between revisions)
(→Growth Curve Of AMB-1 (BBa_K624032))
(→Growth Curve Of AMB-1 (BBa_K624032))
Line 20: Line 20:
[[Image:AMB-1growthcurve.PNG|frame|center|'''AMB-1 growth curve with MSGM.''' <br>
[[Image:AMB-1growthcurve.PNG|frame|center|'''AMB-1 growth curve with MSGM.''' <br>
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.]]
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.]]
-
<font size=3>We present AMB-1's growth curve in OD565, the maximum absorbance of AMB-1. It is important to find out the '''log phase''', because the log phase is the best time for electroporation. Therefore, by keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), we can get the best results for electroporation. After seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. The rate of bacterial growth equalling to death rate is best liky to be achieved in this time. And it is of best quality  to undergo electroporation or further operation.
+
<font size=3>We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. It is important to find out the '''log phase''', because the log phase is the best time for electroporation. Therefore, by keeping AMB-1 in "log phase" (approximately <font color=blue>'''3~6 days'''</font> in Enrich-MSGM, <font color=blue>'''7~10 days'''</font> in MSGM), we can get the best results for electroporation. After seven days, the bacterium would go into '''stationary phase'''. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. The rate of bacterial growth equalling to death rate is best liky to be achieved in this time. And it is of best quality  to undergo electroporation or further operation.
</font>
</font>

Revision as of 03:06, 6 October 2011

Slide Down Box Menu with jQuery and CSS3






Contents

"Magnetotaxis": Chassis Page

Chassis page titled "[http://partsregistry.org/Magnetotaxis Magnetotaxis]" has been created in Parts Registry, please do visit this site for useful more information related to this species and the newly established chassis.

Growth Curve Of AMB-1 (BBa_K624032)

AMB-1 growth curve with MSGM.
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.

We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. It is important to find out the log phase, because the log phase is the best time for electroporation. Therefore, by keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), we can get the best results for electroporation. After seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. The rate of bacterial growth equalling to death rate is best liky to be achieved in this time. And it is of best quality to undergo electroporation or further operation.

pYMB Test Result

International Collaboration Reports

One of our collaborator, Tsinghua has claimed pYMBG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K624021 BBa_K624021]) as an effective shuttle vector.

Retrieved from "http://2011.igem.org/Team:NYMU-Taipei/results/chassis1"